Anti-TRIB2 antibody - N-terminal (ab175879)

Overview

  • Product name
    Anti-TRIB2 antibody - N-terminal
    See all TRIB2 primary antibodies
  • Description
    Rabbit polyclonal to TRIB2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human TRIB2 aa 6-35 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q92519

  • Positive control
    • 293 cell lysate, transiently transfected with the TRIB2 gene; Mouse brain tissue; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 39 kDa.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling TRIB2 with ab175879 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-TRIB2 antibody - N-terminal (ab175879) at 1/1000 dilution

    Lane 1 : 293 cell lysate, non-transfected
    Lane 2 : 293 cell lysate, transiently transfected with the TRIB2 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 39 kDa

  • Flow cytometric analysis of 293 cells labeling TRIB2 with ab175879 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175879 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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