Key features and details
- Rabbit polyclonal to TRIB3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIB3 antibody
See all TRIB3 primary antibodies
DescriptionRabbit polyclonal to TRIB3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 174-358 of Human TRIB3 (NP_066981.2).
- HepG2, HUVEC, HUVEC (treat with Sphingosine 1-phosphate) whole cell lysate, HeLa cells, NCIN87 Xenograft tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionDisrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Tissue specificityHighest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
Anti-TRIB3 antibody (ab137526) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
Anti-TRIB3 antibody (ab137526) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg
Predicted band size: 40 kDa
Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labelling TRIB3 with ab137526 at 1/100.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling TRIB3 with ab137526 at 1/200. The lower panel shows costaining with Hoechst 33342.
ab137526 has been referenced in 3 publications.
- Hong B et al. TRIB3 Promotes the Proliferation and Invasion of Renal Cell Carcinoma Cells via Activating MAPK Signaling Pathway. Int J Biol Sci 15:587-597 (2019). PubMed: 30745845
- Choi J et al. Involvement of endoplasmic reticulum stress in regulation of endometrial stromal cell invasiveness: possible role in pathogenesis of endometriosis. Mol Hum Reprod 25:101-110 (2019). PubMed: 30657961
- Choi JY et al. Ovarian steroid dependence of endoplasmic reticulum stress involvement in endometrial cell apoptosis during the human endometrial cycle. Reproduction 155:493-503 (2018). PubMed: 29764928