Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 174-358 of Human TRIB3 (NP_066981.2).

  • Positive control

    • HepG2, HUVEC, HUVEC (treat with Sphingosine 1-phosphate) whole cell lysate, HeLa cells, NCIN87 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
  • Tissue specificity

    Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C20orf97 antibody
    • Neuronal cell death inducible putative kinase antibody
    • Neuronal cell death-inducible putative kinase antibody
    • NIPK antibody
    • p65 interacting inhibitor of NF-kappaB antibody
    • p65-interacting inhibitor of NF-kappa-B antibody
    • SINK antibody
    • SKIP 3 antibody
    • SKIP3 antibody
    • TRB 3 antibody
    • TRB-3 antibody
    • TRB3 antibody
    • TRIB 3 antibody
    • Trib3 antibody
    • TRIB3_HUMAN antibody
    • Tribbles homolog 3 antibody
    • Tribbles pseudokinase 3 antibody
    • Tribbles3 antibody
    see all

Images

  • Anti-TRIB3 antibody (ab137526) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Anti-TRIB3 antibody (ab137526) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labelling TRIB3 with ab137526 at 1/100.
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling TRIB3 with ab137526 at 1/200. The lower panel shows costaining with Hoechst 33342.

References

This product has been referenced in:

  • Hong B  et al. TRIB3 Promotes the Proliferation and Invasion of Renal Cell Carcinoma Cells via Activating MAPK Signaling Pathway. Int J Biol Sci 15:587-597 (2019). Read more (PubMed: 30745845) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (4-15)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 09 2019

Application
Immunoprecipitation
Immuno-precipitation step
Other - dynabeads protein A
Sample
Human Cell lysate - whole cell (breast cancer)
Specification
breast cancer
Total protein in input
100 µg

Abcam user community

Verified customer

Submitted Mar 26 2015

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