Anti-TRIB3 antibody (ab137526)
Key features and details
- Rabbit polyclonal to TRIB3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRIB3 antibody
See all TRIB3 primary antibodies -
Description
Rabbit polyclonal to TRIB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 174-358 of Human TRIB3 (NP_066981.2).
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Positive control
- WB: Wild-type HCT, HepG2, HUVEC and HUVEC (treat with Sphingosine 1-phosphate) whole cell lysates. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. -
Tissue specificity
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57761 Human
- Omim: 607898 Human
- SwissProt: Q96RU7 Human
- Unigene: 516826 Human
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Alternative names
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
Images
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All lanes : Anti-TRIB3 antibody (ab137526) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 cell lysate
Lane 2 : TRIB3 knockout HCT 116 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDaFalse colour image of Western blot: Anti-TRIB3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137526 was shown to bind specifically to TRIB3. A band was observed at 40 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in TRIB3 knockout cell line ab273718 (knockout cell lysate ab275249). To generate this image, wild-type and TRIB3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Anti-TRIB3 antibody (ab137526) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Anti-TRIB3 antibody (ab137526) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg
Predicted band size: 40 kDa -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling TRIB3 with ab137526 at 1/200. The lower panel shows costaining with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab137526 has been referenced in 13 publications.
- Hao N et al. Laminin-integrin a6b4 interaction activates notch signaling to facilitate bladder cancer development. BMC Cancer 22:558 (2022). PubMed: 35585515
- Haraguchi-Suzuki K et al. Local anesthetic lidocaine-inducible gene, growth differentiation factor-15 suppresses the growth of cancer cell lines. Sci Rep 12:14520 (2022). PubMed: 36008442
- Shen P et al. TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway. Exp Ther Med 21:313 (2021). PubMed: 33717256
- Bao XY et al. TRIB3 promotes proliferation, migration, and invasion of retinoblastoma cells by activating the AKT/mTOR signaling pathway. Cancer Biomark 31:307-315 (2021). PubMed: 33896816
- Wang RQ et al. Tribbles pseudokinase 3 (TRIB3) contributes to the progression of hepatocellular carcinoma by activating the mitogen-activated protein kinase pathway. Ann Transl Med 9:1253 (2021). PubMed: 34532390