Product nameAnti-TRIB3 antibody [EPR3150]
See all TRIB3 primary antibodies
DescriptionRabbit monoclonal [EPR3150] to TRIB3
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human TRIB3 aa 50-150. The exact sequence is proprietary.
- 293T lysates, untreated or treated with Ionomycin and TPA.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
FunctionDisrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Tissue specificityHighest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
All lanes : Anti-TRIB3 antibody [EPR3150] (ab75774) at 1/1000 dilution
Lane 1 : 293T lysates, treated with Ionomycin and
Lane 2 : 293T lysates, untreated
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
ab75774 has not yet been referenced specifically in any publications.