Overview

  • Product name

    Anti-TRIB3 antibody [EPR3151Y]
    See all TRIB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3151Y] to TRIB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIB3 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T cell lysates, untreated and treated with Ionomycin and TPA.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
    • Tissue specificity

      Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C20orf97 antibody
      • Neuronal cell death inducible putative kinase antibody
      • Neuronal cell death-inducible putative kinase antibody
      • NIPK antibody
      • p65 interacting inhibitor of NF-kappaB antibody
      • p65-interacting inhibitor of NF-kappa-B antibody
      • SINK antibody
      • SKIP 3 antibody
      • SKIP3 antibody
      • TRB 3 antibody
      • TRB-3 antibody
      • TRB3 antibody
      • TRIB 3 antibody
      • Trib3 antibody
      • TRIB3_HUMAN antibody
      • Tribbles homolog 3 antibody
      • Tribbles pseudokinase 3 antibody
      • Tribbles3 antibody
      see all

    Images

    • All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

      Lane 1 : 293T cell lysates, treated with Ionomycin and
      TPA
      Lane 2 : 293T cell lysates, untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    References

    This product has been referenced in:

    • Hong B  et al. TRIB3 Promotes the Proliferation and Invasion of Renal Cell Carcinoma Cells via Activating MAPK Signaling Pathway. Int J Biol Sci 15:587-597 (2019). Read more (PubMed: 30745845) »
    • Westrich JA  et al. Human Papillomavirus 16 E7 Stabilizes APOBEC3A Protein by Inhibiting Cullin 2-Dependent Protein Degradation. J Virol 92:N/A (2018). Read more (PubMed: 29367246) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cell lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    35 µg
    Specification
    human cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jul 31 2019

    Answer

    Thank you for contacting us.  Both of these anti-TRIB3 antibodies has been tested for immunoprecipiation and are guaranteed for its use in this application. They have not been tested side by side and so we have no data on whether one may work better for Co-IP than the other. The immunogen sequence for ab50516 is GSLSRKKRLELDDNLDTER and is aa 11-29. The immunogen sequence for ab75846 is considered propreitary and cannot be given out, although it is a small peptide from the C-terminus.  Both antibodies are tested and guaranteed in human. ab50516 has not been tested in mouse. ab75846 has been tested in mouse and DOES NOT react. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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