Key features and details
- Mouse monoclonal [AE15] to Trichohyalin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Trichohyalin antibody [AE15]
See all Trichohyalin primary antibodies
DescriptionMouse monoclonal [AE15] to Trichohyalin
SpecificityThis antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Urea-mercaptoethanol extract of human hair follicles.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab58755 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 220 kDa.|
FunctionIntermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
Tissue specificityFound in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.
Sequence similaritiesBelongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Developmental stageExpressed during late differentiation of the epidermis.
DomainConsists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species.
modificationsSubstrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase.
- Information by UniProt
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
Anti-Trichohyalin antibody [AE15] (ab58755) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 165 kDa. We are unsure as to the identity of these extra bands.
ab58755 has been referenced in 14 publications.
- Tripurani SK et al. Suppression of Wnt/ß-catenin signaling by EGF receptor is required for hair follicle development. Mol Biol Cell 29:2784-2799 (2018). PubMed: 30188763
- Sardella C et al. Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion. J Invest Dermatol 138:500-510 (2018). PubMed: 28964716
- Leng X et al. Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay". Exp Dermatol N/A:N/A (2018). PubMed: 30033549
- Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rabbit . PubMed: 28190929
- Imsland F et al. Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses. Nat Genet 48:152-8 (2016). PubMed: 26691985