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The Trichrome Stain (Connective Tissue Stain) is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
|Nuclei||Dark Red to Black/Blue|
Control Tissue: Lung, Uterus, Small Intestine, Stomach
|Components||100 tests||100 tests|
|Acetic Acid Solution||1 x 125ml||1 x 125ml|
|Aniline Blue Solution||1 x 125ml||1 x 125ml|
|Biebrich Scarlet/Acid Fuchsin Solution||1 x 125ml||1 x 125ml|
|Bouin's Fluid||1 x 125ml||1 x 125ml|
|Phosphomolybdic/Phosphotungstic acid solution||1 x 125ml||1 x 125ml|
|Weigert's Iron, Hematoxylin (A)||1 x 125ml||1 x 125ml|
|Weigert's Iron, Hematoxylin (B)||1 x 125ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab150686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Histology||Use at an assay dependent concentration.|
Ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
A paraffin-embedded section of healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60ºC for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60ºC in humid conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"