For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    trichrome-stain-kit-connective-tissue-stain-ab150686.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits IHC tools Counterstains and Special stains Trichrome Stain
Share by email

Trichrome Stain Kit (Connective Tissue Stain) (ab150686)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (1)References (89)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

You may also be interested in

Biochemical
Product image
Biebrich scarlet, Azo organosulfur dye (ab146312)
Primary
Product image
Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)
ELISA
Product image
Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)

View more associated products

Overview

  • Product name

    Trichrome Stain Kit (Connective Tissue Stain)
  • Product overview

    Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.


    The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.


    Trichrome stain protocol summary:
    - deparaffinize sections if necessary
    - preheat Bouin's Fluid to 54-64ºC
    - incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
    - rinse in water
    - incubate slide in Weigert's Iron Hematoxylin for 5 min
    - rinse in water
    - incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
    - rinse in water
    - differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
    - incubate slide in Aniline Blue solution for 5-10 min
    - rinse in water
    - incubate slide in acetic acid solution for 3-5 min
    - dehydrate, clear and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Collagen Blue
    Muscle Fibers    Red
    Nuclei Dark Red to Black/Blue

    Control Tissue: Lung, Uterus, Small Intestine, Stomach.

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 100 tests
    Acetic Acid Solution (1%) 1 x 125ml
    Aniline Blue Solution 1 x 125ml
    Biebrich Scarlet/Acid Fuchsin Solution 1 x 125ml
    Bouin's Fluid 1 x 125ml
    Phosphomolybdic/Phosphotungstic acid solution 1 x 125ml
    Weigert's Iron, Hematoxylin (A) 1 x 125ml
    Weigert's Iron, Hematoxylin (B) 1 x 125ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Counterstains and Special stains
    • Trichrome Stain

Images

  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
    ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

    ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.

  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
    ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

    ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
    ab150686 - Trichrome Stain Kit (Connective Tissue Stain)This image is courtesy of an Abreview submitted by Javier Rodriguez

    A paraffin-embedded section of  healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (89)

Publishing research using ab150686? Please let us know so that we can cite the reference in this datasheet.

ab150686 has been referenced in 89 publications.

  • Moon YJ  et al. ß-Cyclodextrin/Triclosan Complex-Grafted Methacrylated Glycol Chitosan Hydorgel by Photocrosslinking via Visible Light Irradiation for a Tissue Bio-Adhesive. Int J Mol Sci 22:N/A (2021). PubMed: 33445775
  • Liu M  et al. Amylin deposition activates HIF1a and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol 4:188 (2021). PubMed: 33580152
  • Huang J  et al. Role of tubular epithelial arginase-II in renal inflammaging. NPJ Aging Mech Dis 7:5 (2021). PubMed: 33654066
  • Almatroodi SA  et al. 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics 13:N/A (2021). PubMed: 33670981
  • Kim YS  et al. The adipokine Retnla deficiency increases responsiveness to cardiac repair through adiponectin-rich bone marrow cells. Cell Death Dis 12:307 (2021). PubMed: 33753732
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Mouse liver - Really easy to use

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
We used this kit to stain mouse liver.
This kit is really easy to use and produces excellent results.
The kit provides all that you need for the staining and the drop on method will save you some reagent and allow you to use it further than the 100 slides advertised.
Some timing troubleshooting might be needed for best results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 17 2022

GOOD KIT, it works well and everything is provided

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
GOOD KIT, it works well and everything is provided. The kit has all the reagents for the procedure. You only need to have Xylene and Ethanol and the basic for miscopy (slides etc.).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Francesco Elia Marino

Verified customer

Submitted Jan 31 2018

Mouse spleen stain

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The sample is a section of parafin mouse spleen in a healthy state. The sample have been deparafined by heating it up to 60ºC during 1 hour and then following a standard deparafination protocol until H2O, then fixed with Bouin's solution (included in the kit) 1 hour at 60ºC in humid conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Javier Rodriguez

Verified customer

Submitted Feb 28 2014

Human xenograft Ewin Sarcoma

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
I have used this kit to stain samples of a Xenograft of Ewin sarcoma cells within a swiss nude mice in order to detect the collagen I fibers present in the tumour bulk.
The kit is ready to use and easily handling.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Javier Rodriguez

Verified customer

Submitted Jan 29 2014

Question

When I was reading your online protocol of the masson trichrome stain, a question came to my mind. Is this also usable for the staining of human connective tissue?

Read More

Abcam community

Verified customer

Asked on Apr 29 2014

Answer

The product ab150686 can be used to stain connective tissues irrespective of species. It will be fine for human samples.

Read More

Padamjeet Singh

Abcam Scientific Support

Answered on Apr 29 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.