Trichrome Stain Kit (Connective Tissue Stain) (ab150686)
- Datasheet
- References (26)
- Protocols
Overview
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Product name
Trichrome Stain Kit (Connective Tissue Stain) -
Product overview
Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.
The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.
Trichrome stain protocol summary:
- deparaffinize sections if necessary
- preheat Bouin's Fluid to 54-64ºC
- incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
- rinse in water
- incubate slide in Weigert's Iron Hematoxylin for 5 min
- rinse in water
- incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
- rinse in water
- differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
- incubate slide in Aniline Blue solution for 5-10 min
- rinse in water
- incubate slide in acetic acid solution for 3-5 min
- dehydrate, clear and mount
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Collagen Blue Muscle Fibers Red Nuclei Dark Red to Black/Blue Control Tissue: Lung, Uterus, Small Intestine, Stomach.
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 100 tests Acetic Acid Solution (1%) 1 x 125ml Aniline Blue Solution 1 x 125ml Biebrich Scarlet/Acid Fuchsin Solution 1 x 125ml Bouin's Fluid 1 x 125ml Phosphomolybdic/Phosphotungstic acid solution 1 x 125ml Weigert's Iron, Hematoxylin (A) 1 x 125ml Weigert's Iron, Hematoxylin (B) 1 x 125ml -
Research areas
Images
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)This image is courtesy of an Abreview submitted by Javier RodriguezA paraffin-embedded section of healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.
Protocols
References
This product has been referenced in:
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
- Ponticorvo A et al. Evaluating clinical observation versus Spatial Frequency Domain Imaging (SFDI), Laser Speckle Imaging (LSI) and thermal imaging for the assessment of burn depth. Burns 45:450-460 (2019). Read more (PubMed: 30327232) »
