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Trichrome Stain Kit (Connective Tissue Stain) (ab150686)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3)Q&A (1)References (38)

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ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
  • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

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Overview

  • Product name

    Trichrome Stain Kit (Connective Tissue Stain)
  • Product overview

    Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.


    The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.


    Trichrome stain protocol summary:
    - deparaffinize sections if necessary
    - preheat Bouin's Fluid to 54-64ºC
    - incubate slide in preheated Bouin's Fluid for 60 min and cool for 10 min
    - rinse in water
    - incubate slide in Weigert's Iron Hematoxylin for 5 min
    - rinse in water
    - incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
    - rinse in water
    - differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
    - incubate slide in Aniline Blue solution for 5-10 min
    - rinse in water
    - incubate slide in acetic acid solution for 3-5 min
    - dehydrate, clear and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Collagen Blue
    Muscle Fibers    Red
    Nuclei Dark Red to Black/Blue

    Control Tissue: Lung, Uterus, Small Intestine, Stomach.

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 100 tests
    Acetic Acid Solution (1%) 1 x 125ml
    Aniline Blue Solution 1 x 125ml
    Biebrich Scarlet/Acid Fuchsin Solution 1 x 125ml
    Bouin's Fluid 1 x 125ml
    Phosphomolybdic/Phosphotungstic acid solution 1 x 125ml
    Weigert's Iron, Hematoxylin (A) 1 x 125ml
    Weigert's Iron, Hematoxylin (B) 1 x 125ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Counterstains and Special stains
    • Trichrome Stain

Associated products

    Images

    • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
      ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

      ab150686 Trichrome Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human muscle.

    • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
      ab150686 - Trichrome Stain Kit (Connective Tissue Stain)

      ab150686 (Trichrome Stain) staining collagen (blue) and muscle fibre (red) in formalin fixed paraffin embedded normal human placenta. Nuclei are counterstained with Weigert’s hematoxylin (blue)

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • ab150686 - Trichrome Stain Kit (Connective Tissue Stain)
      ab150686 - Trichrome Stain Kit (Connective Tissue Stain)This image is courtesy of an Abreview submitted by Javier Rodriguez

      A paraffin-embedded section of  healthy mouse spleen stained with ab150686 - Trichrome Stain Kit (Connective Tissue Stain). The sample was deparaffinized by heating to 60°C for 1 hour and then deparaffinization following a standard protocol. Sections were then fixed with Bouin's solution (included in the kit) for 1 hour at 60°C in humid conditions.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (38)

    Publishing research using ab150686? Please let us know so that we can cite the reference in this datasheet.

    ab150686 has been referenced in 38 publications.

    • Zhou H  et al. Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing. Mol Metab 36:100971 (2020). PubMed: 32246911
    • Meng G  et al. Dexamethasone Attenuates X-Ray-Induced Activation of the Autotaxin-Lysophosphatidate-Inflammatory Cycle in Breast Tissue and Subsequent Breast Fibrosis. Cancers (Basel) 12:N/A (2020). PubMed: 32325715
    • Wang Y  et al. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. Elife 9:N/A (2020). PubMed: 32479261
    • Xie G  et al. Serum metabolite profiles are associated with the presence of advanced liver fibrosis in Chinese patients with chronic hepatitis B viral infection. BMC Med 18:144 (2020). PubMed: 32498677
    • Li S  et al. hsa_circ_0004018 suppresses the progression of liver fibrosis through regulating the hsa-miR-660-3p/TEP1 axis. Aging (Albany NY) 12:11517-11529 (2020). PubMed: 32584784
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    GOOD KIT, it works well and everything is provided

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    GOOD KIT, it works well and everything is provided. The kit has all the reagents for the procedure. You only need to have Xylene and Ethanol and the basic for miscopy (slides etc.).

    Dr. Francesco Elia Marino

    Verified customer

    Submitted Jan 31 2018

    Question

    When I was reading your online protocol of the masson trichrome stain, a question came to my mind. Is this also usable for the staining of human connective tissue?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 29 2014

    Answer

    The product ab150686 can be used to stain connective tissues irrespective of species. It will be fine for human samples.

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    Answered on Apr 29 2014

    Mouse spleen stain

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The sample is a section of parafin mouse spleen in a healthy state. The sample have been deparafined by heating it up to 60ºC during 1 hour and then following a standard deparafination protocol until H2O, then fixed with Bouin's solution (included in the kit) 1 hour at 60ºC in humid conditions.

    Mr. Javier Rodriguez

    Verified customer

    Submitted Feb 28 2014

    Human xenograft Ewin Sarcoma

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    I have used this kit to stain samples of a Xenograft of Ewin sarcoma cells within a swiss nude mice in order to detect the collagen I fibers present in the tumour bulk.
    The kit is ready to use and easily handling.

    Mr. Javier Rodriguez

    Verified customer

    Submitted Jan 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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