Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 143-158 of Mouse TRIF.

  • Positive control
    • WB: Mouse spleen, intestine, liver and hepatocytes lysates; Raji and RAW 264.7 cell lysates. IHC-P: Human colon tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa.
ICC/IF 1/10 - 1/100.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
  • Tissue specificity
    Ubiquitously expressed but with higher levels in liver.
  • Sequence similarities
    Contains 1 TIR domain.
  • Domain
    The N-terminal region is essential for activation of the IFNB promoter activity.
  • Post-translational
    modifications
    Phosphorylated by TBK1.
  • Information by UniProt
  • Database links
  • Alternative names
    • IIAE6 antibody
    • MGC35334 antibody
    • MyD88 3 antibody
    • Proline rich vinculin and TIR domain containing protein B antibody
    • Proline-rich antibody
    • PRVTIRB antibody
    • Putative NF kappa B activating protein 502H antibody
    • Putative NF-kappa-B-activating protein 502H antibody
    • Putative NFkB activating protein antibody
    • TCAM1_HUMAN antibody
    • TICAM 1 antibody
    • TICAM-1 antibody
    • Ticam1 antibody
    • TIR domain containing adapter molecule 1 antibody
    • TIR domain containing adapter protein inducing IFN beta antibody
    • TIR domain containing adaptor inducing interferon beta antibody
    • TIR domain-containing adapter molecule 1 antibody
    • TIR domain-containing adapter protein inducing IFN-beta antibody
    • Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody
    • Toll like receptor adaptor molecule 1 antibody
    • Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody
    • TRIF antibody
    • TRIF protein antibody
    • vinculin and TIR domain-containing protein B antibody
    see all

Images

  • Anti-TRIF antibody (ab13810) at 1/1000 dilution + Mouse spleen lysate
  • ab13810 (2µg/ml) staining TRIF in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of mucosal cell cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate with immunizing peptide
    Lane 2 : Mouse spleen lysate
  • All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution

    Lane 1 : Mouse intestine lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
  • Anti-TRIF antibody (ab13810) at 1/100 dilution + Raji (human Burkitt's lymphoma cell line) cell lysate
  • A431 (human epidermoid carcinoma cell line) cells stained for TRIF (green) using ab13810 at 1/10 dilution in ICC/IF with a Dylight 488 secondary antibody. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

  • All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse hepatocytes lysate

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/10000 dilution

    See Abreview

References

This product has been referenced in:
  • Wang Y  et al. Sparstolonin B improves neurological outcomes following intracerebral hemorrhage in mice. Exp Ther Med 15:5436-5442 (2018). Read more (PubMed: 29844805) »
  • Liang S  et al. TLR2 and TLR3 expression as a biomarker for the risk of doxorubicin-induced heart failure. Toxicol Lett 295:205-211 (2018). Read more (PubMed: 29959987) »
See all 25 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 10 2014

Answer

Thank you for letting me know that the antibody reached you safely. The colour of antibodies does vary slightly depending on how they were purified and the buffer which they are stored in. ab13810 is a preparation of whole antiserum from rabbit and it may be that the other proteins and compounds present in the serum are contributing to the slightly yellow colour. It should not however change the activity of the antibody. I would suggesttrying theantibody as usual and if you have any problems please do let us know. Please make sure that it is stored as indicated on the datasheet: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. If you could please fill in the form I have attached that will allow me to more fully understand the procedure you have been carrying out and highlight any areas where I may be able to help you optimise the performance of the antibody. This will also allow me to investigate this complaint internally and initiate any additional testing that may be required. If you could include some images of your results that would be very helpful. Many thanks for your continued cooperation.

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Answer

Thank you for the swift reply. I have arranged for a free of charge replacement of a different lot to be sent to you directly (Order number:xxxxx number:xxxxxx). This should be with you tomorrow. I would be very grateful if you could find the time to answer the additional questions which I sent so that we can more fully understand the problems you have been experiencing and initiate any investigation into the matter which may be necessary. Again, I am sorry for the inconvenience which this problem has caused and hope the replacement vial will bring better results. I look forward to receiving your reply.

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Question
Answer

Thank you for contacting us again. I am very sorry that the email we tried to send did not reach you. As discussed over the phone these are the details I will need in order to organise the replacement for you: Lot number Purchase order number or preferably Abcam order number: Or delivery address andapproximatedate of order: If you could get this information back to me before 3:30 pm today the replacement should be with you tomorrow. If you could also answer the following questions when you have time it would be much appreciated. This information allows us to monitor the quality of our products and instigate any further testing where it might be required. General Information Antibody storage conditions (temperature/reconstitution etc) Description of the problem (high background, wrong band size, more bands, no band etc.) Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) Amount of protein loaded Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) Transfer and blocking conditions (Buffer/time period, Blocking agent etc.) Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step) Detection method (ECL, ECLPlus etc.) Positive and negative controls used (please specify) Optimization attempts (problem solving) How many times have you tried the Western? Have you run a "No Primary" control? Yes No Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place? What steps have you altered? Additional Notes: Image: I apologize again for the delay in resolving this matter and thank you for your patience in this matter. I look forward to receiving your reply.

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Application
Western blot
Sample
Human Cell lysate - whole cell (macrophages)
Loading amount
25000 cells
Specification
macrophages
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 29 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (THP1 human monocytes)
Loading amount
15 µg
Specification
THP1 human monocytes
Treatment
LPS time course
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted May 30 2007

Question
Answer

Thank you for your enquiry. I have been in contact with the originator of this antibody, and they used mouse spleen from an adult Balb/c, approximately 6 months old. With that knowledge, I would recommend the 14 day old lysate over the 7 day old lysate. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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