Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab205363 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab205363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 76 kDa).|
FunctionInvolved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
Tissue specificityUbiquitously expressed but with higher levels in liver.
Sequence similaritiesContains 1 TIR domain.
DomainThe N-terminal region is essential for activation of the IFNB promoter activity.
modificationsPhosphorylated by TBK1.
- Information by UniProt
- IIAE6 antibody
- MGC35334 antibody
- MyD88 3 antibody
Anti-TRIF antibody (ab205363) at 2 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
This product has been referenced in:
- Chen W et al. Effects of restrictions on maternal feed intake on the immune indexes of umbilical cord blood and liver Toll-like receptor signaling pathways in fetal goats during pregnancy. J Anim Sci Biotechnol 10:29 (2019). Read more (PubMed: 31011422) »