Overview

  • Product name

  • Description

    Goat polyclonal to TRIF
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human TRIF aa 422-434 (internal sequence). (NP_891549.1).
    Sequence:

    PDGATFCEDFQVP


    Database link: Q8IUC6

  • Positive control

    • Daudi cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab205363 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 76 kDa).

Target

  • Function

    Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
  • Tissue specificity

    Ubiquitously expressed but with higher levels in liver.
  • Sequence similarities

    Contains 1 TIR domain.
  • Domain

    The N-terminal region is essential for activation of the IFNB promoter activity.
  • Post-translational
    modifications

    Phosphorylated by TBK1.
  • Information by UniProt
  • Database links

  • Alternative names

    • IIAE6 antibody
    • MGC35334 antibody
    • MyD88 3 antibody
    • Proline rich vinculin and TIR domain containing protein B antibody
    • Proline-rich antibody
    • PRVTIRB antibody
    • Putative NF kappa B activating protein 502H antibody
    • Putative NF-kappa-B-activating protein 502H antibody
    • Putative NFkB activating protein antibody
    • TCAM1_HUMAN antibody
    • TICAM 1 antibody
    • TICAM-1 antibody
    • Ticam1 antibody
    • TIR domain containing adapter molecule 1 antibody
    • TIR domain containing adapter protein inducing IFN beta antibody
    • TIR domain containing adaptor inducing interferon beta antibody
    • TIR domain-containing adapter molecule 1 antibody
    • TIR domain-containing adapter protein inducing IFN-beta antibody
    • Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody
    • Toll like receptor adaptor molecule 1 antibody
    • Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody
    • TRIF antibody
    • TRIF protein antibody
    • vinculin and TIR domain-containing protein B antibody
    see all

Images

  • Anti-TRIF antibody (ab205363) at 2 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 76 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

This product has been referenced in:

  • Chen W  et al. Effects of restrictions on maternal feed intake on the immune indexes of umbilical cord blood and liver Toll-like receptor signaling pathways in fetal goats during pregnancy. J Anim Sci Biotechnol 10:29 (2019). Read more (PubMed: 31011422) »
See 1 Publication for this product

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