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  1. Link

    triglyceride-assay-kit-fluorometric-reducing-samples-ab178780.pdf

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Triglyceride Assay Kit (Fluorometric, Reducing Samples) (ab178780)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (1)References (5)

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Triglyceride Standard Curve
  • Measurement of triglyceride levels in samples

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Adherent cells, Plasma, Saliva, Serum, Suspension cells, Tissue
  • Sensitivity: 0.4 µM

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Overview

  • Product name

    Triglyceride Assay Kit (Fluorometric, Reducing Samples)
    See all Triglyceride kits
  • Detection method

    Fluorescent
  • Sample type

    Saliva, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    < 0.4 µM
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (ab65336).


    In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ed/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.


    This assay is simple, sensitive and easy to use and is high-throughput (HTP) adaptable.


    Triglyceride assay protocol summary:
    - add samples and standards to wells
    - add lipase and incubate for 20 min
    - add reaction mix and incubate for 30 min
    - analyze with a microplate reader.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K614 PicoProbe™ Triglyceride (TG) Fluorometric Assay Kit. K614-100 is the same size as the 100 test size of ab178780.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Lipase Blue cap/ Clear vial 1 vial
    PicoProbe Blue cap/Amber vial 1 x 0.4ml
    Triglyceride Assay Buffer 1 x 25ml
    Triglyceride Developer Red 1 vial
    Triglyceride Enzyme Mix (lyophilized) Green 1 vial
    Triglyceride Standard Yellow 1 x 0.3ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Fatty Acid Assays
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Relevance

    Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
  • Alternative names

    • TG
    • Triacylglyceride
    • triglycerides

Images

  • Triglyceride Standard Curve
    Triglyceride Standard Curve

    Example of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.

  • Measurement of triglyceride levels in samples
    Measurement of triglyceride levels in samples

    Mesurament of TG levels in rat liver (~15 µg), human serum (1 µL) and saliva (10 µL). Assays were performed according to kit protocol.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab178780? Please let us know so that we can cite the reference in this datasheet.

ab178780 has been referenced in 5 publications.

  • Panajatovic MV  et al. Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1a Overexpression. Int J Mol Sci 22:N/A (2021). PubMed: 34066911
  • Zhu X  et al. MyD88 signalling is critical in the development of pancreatic cancer cachexia. J Cachexia Sarcopenia Muscle 10:378-390 (2019). PubMed: 30666818
  • Driescher N  et al. The impact of sugar-sweetened beverage intake on rat cardiac function. Heliyon 5:e01357 (2019). PubMed: 30949605
  • Yisireyili M  et al. Chronic restraint stress induces esophageal fibrosis with enhanced oxidative stress in a murine model. Exp Ther Med 18:1375-1383 (2019). PubMed: 31316626
  • do Carmo JM  et al. Impact of leptin deficiency compared with neuronal-specific leptin receptor deletion on cardiometabolic regulation. Am J Physiol Regul Integr Comp Physiol 317:R552-R562 (2019). PubMed: 31411897

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Triglyceride Quantification of Mouse Feces

Average Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
In order to prepare the feces, I followed the protocol given in the "ab65336 Triglyceride Quantification Assay kit (Colorimetric/Fluorometric)" for tissue samples, with a few slight variations:
-Grind snap frozen feces with a mortar and pestle (initial reccomendation= 20 mg)
-Resuspend and homogenize in 1 mL of 5%NP-40/ddH2O solution.
-Slowly heat the samples to 80 – 100°C in a water bath for 2 – 5 minutes or until the NP-40 becomes cloudy, then cool down to room temperature.
-Repeat the heating one more time to solubilize all triglyceride.
-Centrifuge for 2 minutes at top speed using a microcentrifuge to remove any insoluble material.
After this preparation, I followed the protocol given with the kit. The results look strange, however I believe that is due to using too low of a concentration of feces.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katherine Rosalie Pelz

Verified customer

Submitted Jul 19 2017

Question

What is the difference between ab178780 and ab65336?

Read More

Abcam community

Verified customer

Asked on Sep 01 2015

Answer

The picoprobe allows ab178780 to be more sensitive than the ab65336. ab178780 detects triglycerides <0.4uM whereas ab65336 can detect 2uM-10mM triglycerides.

I hope this helps!

Read More

Jared Kaminski

Abcam Scientific Support

Answered on Sep 01 2015

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