Triglyceride Assay Kit (Fluorometric, Reducing Samples) (ab178780)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Triglyceride Assay Kit (Fluorometric, Reducing Samples)
See all Triglyceride kits -
Detection method
Fluorescent -
Sample type
Saliva, Serum, Plasma, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
< 0.4 µM -
Assay time
1h 00m -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (ab65336).
In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ed/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.
This assay is simple, sensitive and easy to use and is high-throughput (HTP) adaptable.
Triglyceride assay protocol summary:
- add samples and standards to wells
- add lipase and incubate for 20 min
- add reaction mix and incubate for 30 min
- analyze with a microplate reader. -
Notes
Previously called PicoProbe Triglyceride Quantification Assay Kit (Fluorometric)
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Lipase Blue cap/ Clear vial 1 vial PicoProbe Blue cap/Amber vial 1 x 0.4ml Triglyceride Assay Buffer 1 x 25ml Triglyceride Developer Red 1 vial Triglyceride Enzyme Mix (lyophilized) Green 1 vial Triglyceride Standard Yellow 1 x 0.3ml -
Research areas
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Relevance
Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis. -
Alternative names
- Triacylglyceride
Images
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Triglyceride Standard CurveExample of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.
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Measurement of triglyceride levels in samplesMesurament of TG levels in rat liver (~15 µg), human serum (1 µL) and saliva (10 µL). Assays were performed according to kit protocol.
Protocols
References
This product has been referenced in:
- Zhu X et al. MyD88 signalling is critical in the development of pancreatic cancer cachexia. J Cachexia Sarcopenia Muscle 10:378-390 (2019). Read more (PubMed: 30666818) »
