Overview

  • Product name

    Triglyceride Assay Kit (Fluorometric, Reducing Samples)
    See all Triglyceride kits
  • Detection method

    Fluorescent
  • Sample type

    Saliva, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    < 0.4 µM
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Triglyceride Assay Kit (Fluorometric, Reducing Samples) ab178780 is suitable for measuring triglyceride levels in samples which contain reducing substances that may interfere with oxidase-based assays such as Triglyceride Assay Kit (ab65336).


    In this triglyceride assay protocol, triglycerides are hydrolyzed to glycerol and fatty acid. The glycerol reacts with Triglyceride Enzyme Mix to form an intermediate product which in turn reacts with the probe and developer to generate fluorescence (Ed/Em 535/587 nm). The generated fluorescence is directly proportional to the amount of triglycerides.


    This assay is simple, sensitive and easy to use and is high-throughput (HTP) adaptable.


    Triglyceride assay protocol summary:
    - add samples and standards to wells
    - add lipase and incubate for 20 min
    - add reaction mix and incubate for 30 min
    - analyze with a microplate reader.

  • Notes

    Previously called PicoProbe Triglyceride Quantification Assay Kit (Fluorometric)

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Lipase Blue cap/ Clear vial 1 vial
    PicoProbe Blue cap/Amber vial 1 x 0.4ml
    Triglyceride Assay Buffer 1 x 25ml
    Triglyceride Developer Red 1 vial
    Triglyceride Enzyme Mix (lyophilized) Green 1 vial
    Triglyceride Standard Yellow 1 x 0.3ml
  • Research areas

  • Relevance

    Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
  • Alternative names

    • Triacylglyceride

Images

  • Example of triglyceride standard curve following assay protocol. Please note that this is an example curve and should not be used for experimental purposes.

  • Mesurament of TG levels in rat liver (~15 µg), human serum (1 µL) and saliva (10 µL). Assays were performed according to kit protocol.

Protocols

References

This product has been referenced in:

  • Zhu X  et al. MyD88 signalling is critical in the development of pancreatic cancer cachexia. J Cachexia Sarcopenia Muscle 10:378-390 (2019). Read more (PubMed: 30666818) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The picoprobe allows ab178780 to be more sensitive than the ab65336. ab178780 detects triglycerides <0.4uM whereas ab65336 can detect 2uM-10mM triglycerides.

I hope this helps!

Read More

Triglyceride Quantification of Mouse Feces

Average Excellent 5/5 (Ease of Use)
Abreviews
In order to prepare the feces, I followed the protocol given in the "ab65336 Triglyceride Quantification Assay kit (Colorimetric/Fluorometric)" for tissue samples, with a few slight variations:
-Grind snap frozen feces with a mortar and pestle (initial reccomendation= 20 mg)
-Resuspend and homogenize in 1 mL of 5%NP-40/ddH2O solution.
-Slowly heat the samples to 80 – 100°C in a water bath for 2 – 5 minutes or until the NP-40 becomes cloudy, then cool down to room temperature.
-Repeat the heating one more time to solubilize all triglyceride.
-Centrifuge for 2 minutes at top speed using a microcentrifuge to remove any insoluble material.
After this preparation, I followed the protocol given with the kit. The results look strange, however I believe that is due to using too low of a concentration of feces.

Katherine Rosalie Pelz

Verified customer

Submitted Jul 19 2017

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