Product nameTriglyceride Assay Kit - Quantification
See all Triglyceride kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Sensitivity> 2 µM
Assay time1h 20m
Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Triglyceride assay protocol summary:
- add samples and standards to wells
- add assay buffer and lipase, and incubate for 20 min
- add triglyceride reaction mix and incubate for 60 min
- analyze with microplate reader
Please note: The General Range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) ab178780.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
How other researchers have used Triglyceride Assay Kit ab65336
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: serum1, plasma2, mammary epithelial and mammary cancer cell line lysate3, Huh7.5 hepatocyte-derived cell line lysate4, primary liver cell line lysates5, sebocyte cell culture lysates6
- Mouse: hepatocyte cell lysates7, liver extract8, serum9, plasma10, kidney extracts11, liver tissue and serum12, cardiac tissue extracts13
- Rat: liver tissue extract14, plasma15
References: 1-Huang Y et al. 2019, 2-Wilson et al 2018, 3-Yem MC et al. 2019, 4-Kim D et al. 2018, 5-Boteon Y et al. 2018, 6-Jin S and Lee MY 2018, 8-Brial F et al. 2019, 7-Zhang et al. 2019, 9-Liu J et al. 2018 and Patton A et al. 2018, 10-Fernandes et al. 2018 and Korholz JC et al 2018 and Liang et al. 2018, 11-Ding W et al. 2018, 12-Cui XB et al. 2018, 13-Rohm M et al. 2018, 14-Yu S et al. 2018, 15-Garcia-Ruiz et al. 2018, 16-Wen CA and Ballard JWO 2019
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Lipase Blue 1 vial Triglyceride Assay Buffer 1 x 25ml Triglyceride Enzyme Mix (lyophilized) Green 1 vial Triglyceride Probe (in DMSO, anhydrous) Red 1 x 200µl Triglyceride Standard (1 mM) Yellow 1 x 300µl
RelevanceTriglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD).
Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted
Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.
HepG2 cells were treated with 25 uM Chloroquine for 72h.
Colorimetric triglyceride standard curve using ab65336.
This product has been referenced in:
- Chi Y et al. Gut microbiota dysbiosis correlates with a low-dose PCB126-induced dyslipidemia and non-alcoholic fatty liver disease. Sci Total Environ 653:274-282 (2019). Read more (PubMed: 30412872) »
- Zhang W et al. SIRT1 mediates the role of RNA-binding protein QKI 5 in the synthesis of triglycerides in non-alcoholic fatty liver disease mice via the PPARa/FoxO1 signaling pathway. Int J Mol Med 43:1271-1280 (2019). Read more (PubMed: 30664220) »