Overview

  • Product name
    Triglyceride Assay Kit - Quantification 
    See all Triglyceride kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Sensitivity
    > 2 µM
  • Range
    2 µM - 10000 µM
  • Assay time
    1h 00m
  • Product overview

    Triglyceride Quantification Assay Kit (ab65336) provides a sensitive, easy assay to measure triglyceride (TG) concentration in mammalian samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).


    If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend using PicoProbe Triglyceride Quantification Assay Kit (Fluorometric) (ab178780).


    Visit our FAQs page for tips and troubleshooting.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    This kit also detects monoglycerides and diglycerides.

    Some components of this kit are available for purchasing as standalone reagents if you need more quantities (lipase, enzyme mix, TG standard or TG probe). Please contact our team for more details.

Properties

Associated products

Images

  • Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD). 

  • Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.

    HepG2 cells were treated with 25 uM Chloroquine for 72h.

  • Colorimetric triglyceride standard curve using ab65336.

Protocols

References

This product has been referenced in:
  • García-Ruiz I  et al. Omentectomy Prevents Metabolic Syndrome By Reducing Appetite and Body Weight In A Diet-Induced Obesity Rat Model. Sci Rep 8:1540 (2018). WB . Read more (PubMed: 29367725) »
  • Feng B  et al. Hypothalamic POMC expression is required for peripheral insulin action on hepatic gluconeogenesis through regulating STAT3 in sepsis rats. J Cell Mol Med 22:1696-1707 (2018). Read more (PubMed: 29285858) »

See all 68 Publications for this product

Customer reviews and Q&As

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Abreviews
The protocol is easy to understand and perform.
We determined the TG level in liver tissue to use in subsequent assays.
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Ms. Ji Hye Han

Verified customer

Submitted Jul 30 2018

Comparing a serial dilution of yellow-bellied marmot (Marmota flaviventris) to a standard serial dilution, we determined the quantity of plasma to use in subsequent assays (shown only the greatest dilution fell on the curve).
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Abcam user community

Verified customer

Submitted Jul 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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