Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human TRIM aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88132)

  • Positive control

    • This antibody gave a positive signal in Human Spleen Tissue Lysate and Jurkat Whole Cell Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells.
  • Tissue specificity

    Strongly expressed in thymus, and to a lesser extent in spleen, lymph node and peripheral blood lymphocytes. Present in T-cells and NK cells, but not B-cells (at protein level).
  • Developmental stage

    Strongly expressed in fetal thymus at weeks 17-24 of gestation. Undetectable in bone marrow and fetal liver.
  • Post-translational
    modifications

    Phosphorylated on tyrosines by LCK or FYN upon TCR activation.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSPC062 antibody
    • pp29/30 antibody
    • T cell receptor interacting molecule antibody
    • T-cell receptor-associated transmembrane adapter 1 antibody
    • T-cell receptor-interacting molecule antibody
    • TCRIM antibody
    • TRAT1 antibody
    • TRAT1_HUMAN antibody
    • TRIM antibody
    see all

Images

  • All lanes : Anti-TRIM antibody (ab79782) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

References

ab79782 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Merci de nous avoir contactés.

Anti-AP2M1(ab75995) a été testé sur des lysats de cellules MCF-7 et SKBR-3. Anti- CTLA4 (ab134090) a été testé sur des lysats de cellules HELA, Raji, MOLT4 et K562. Ces deux anticorps me semblent être des très bon choix. Ils sont tous les deux des monoclonaux de lapin et les monoclonaux de lapins sont connus pour donner de bons résultats.

Pour l’anti-TRIM, je vous recommande d’utiliser ab79782 plutôt que ab9529. Ab79782 a été testé sur des lysats de cellules Jurkat et a donné de bons résultats.

Je vous recommande également de charger 10 ug de protéine par puits.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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