Key features and details
- Rabbit polyclonal to TRIM13
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM13 antibody
See all TRIM13 primary antibodies
DescriptionRabbit polyclonal to TRIM13
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TRIM13 aa 40-310.
Database link: O60858
- WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human breast cancer and brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab234847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionE3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger.
DomainThe coiled-coil domain is required for the induction of autophagy during endoplasmic reticulum (ER) stress.
The RING-type zinc finger is required for auto-polyubiquitination.
The C-terminal domain transmembrane domain is indispensable for the localization to the ER.
modificationsAuto-ubiquitinated; requires the RING-type zinc finger. Auto-polyubiquitination leads to proteasomal degradation.
Cellular localizationEndoplasmic reticulum membrane. Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.
- Information by UniProt
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All lanes : Anti-TRIM13 antibody (ab234847) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Paraffin-embedded human breast cancer tissue stained for TRIM13 with ab234847 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for TRIM13 with ab234847 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234847 has been referenced in 1 publication.
- Xu L et al. TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-?B pathway in non-small-cell lung carcinoma cells. Gene 715:144015 (2019). PubMed: 31357025