Product nameAnti-TRIM13 antibody
See all TRIM13 primary antibodies
DescriptionRabbit polyclonal to TRIM13
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to Human TRIM13 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; HEk293 as well as HeLa Nuclear lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab5515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionE3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger.
DomainThe coiled-coil domain is required for the induction of autophagy during endoplasmic reticulum (ER) stress.
The RING-type zinc finger is required for auto-polyubiquitination.
The C-terminal domain transmembrane domain is indispensable for the localization to the ER.
modificationsAuto-ubiquitinated; requires the RING-type zinc finger. Auto-polyubiquitination leads to proteasomal degradation.
Cellular localizationEndoplasmic reticulum membrane. Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.
- Information by UniProt
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Left: Endogenous TRIM13
Detection using indirect fluorescence of the signal corresponding to endogenous TRIM13 in 293T cells. Cells fixed using 4% formaldehyde, blocked with PBS containing 3% milk and 0.5% Triton X-100, incubated for 1 hour at 37 °C using a 1/50 dilution of antibody ab5515. Cells were then washed 3 times and incubated for 1 hour at 37 °C with a goat anti-rabbit secondary antibody (1/500 dilution) coupled to Alexa Fluor 555 (Molecular Probes). Following 3 more washes, cells were stained with DAPI and mounted with Vectashield.
Right: Overexpressed TRIM13
Top: anti-FLAG antibody
Bottom: Merge of anti-FLAG and ab5515 (and DAPI) staining.
Detection using indirect fluorescence of the signal corresponding to staining with anti-FLAG mouse antibody (top) and antibody ab5515 against TRIM13 (middle) in 293T cells. 293T cells were transfected with vectors for overexpression of flagged TRIM13
Detection using indirect fluorescence of the signal corresponding to endogenous TRIM13 in 293T cells. Using Lipofectamine 2000 (Invitrogen), cells were transfected with either a control shRNA directed against luciferase (left, SiRluc) or a specific siRNA directed against TRIM13 (right). 48 hours post-transfection, cells were fixed, blocked, and incubated with a 1/50 dilution of ab5515 at 37 °C for 1 hour. Cells were then washed 3 times and incubated at 37 °C for 1 hour with a 1/500 dilution of goat anti-rabbit antibody coupled with Alexa Fluor 555 (Molecular Probes). Following 3 further washes cells were stained with DAPI and mounted with Vectashield.
Antibody signals were extinguished when a specific RNAi was used. The few cells which retained a signal were presumably not transfected.
Anti-TRIM13 antibody (ab5515) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab5515 has not yet been referenced specifically in any publications.