Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2500. Predicted molecular weight: 50 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInhibits the transcriptional activity of SPI1 in a dose-dependent manner.
Tissue specificityHighest expression in liver; undetectable in skeletal muscle.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
DomainThe B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
- Information by UniProt
- 5830400N10Rik antibody
- KIAA0129 antibody
- pub antibody
All lanes : Anti-TRIM14 antibody (ab185349) at 1/1000 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251MG sp cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate
Lane 4 : Human liver tissue lysate
Predicted band size: 50 kDa
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIM14 with ab185349 at 1/1000 dilution.