Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human TRIM14 aa 86-162.
    Sequence:

    HIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDK NTQLTLQVYREQADSCREQLDIMNDLS


    Database link: Q14142

  • Positive control

    • Human liver tissue lysate; Human liver tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Predicted molecular weight: 50 kDa.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Inhibits the transcriptional activity of SPI1 in a dose-dependent manner.
  • Tissue specificity

    Highest expression in liver; undetectable in skeletal muscle.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
  • Domain

    The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5830400N10Rik antibody
    • KIAA0129 antibody
    • pub antibody
    • TRI14_HUMAN antibody
    • TRIM14 antibody
    • tripartite motif protein 14 antibody
    • tripartite motif protein TRIM14 antibody
    • tripartite motif-containing 14 antibody
    • Tripartite motif-containing protein 14 antibody
    see all

Images

  • All lanes : Anti-TRIM14 antibody (ab185349) at 1/1000 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251MG sp cell lysate
    Lane 3 : Human plasma (IgG/HSA depleted) lysate
    Lane 4 : Human liver tissue lysate

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIM14 with ab185349 at 1/1000 dilution.

References

This product has been referenced in:

  • Feng S  et al. Tripartite motif-containing 14 (TRIM14) promotes epithelial-mesenchymal transition via ZEB2 in glioblastoma cells. J Exp Clin Cancer Res 38:57 (2019). Read more (PubMed: 30728039) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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