Anti-TRIM16 antibody (ab72129)
Key features and details
- Rabbit polyclonal to TRIM16
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM16 antibody
See all TRIM16 primary antibodies -
Description
Rabbit polyclonal to TRIM16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human TRIM16, NP_006461.3.
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Positive control
- Whole cell lysate from Hela, 293T and NIH 3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
May play a role in the regulation of keratinocyte differentiation. -
Tissue specificity
Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10626 Human
- Entrez Gene: 94092 Mouse
- Entrez Gene: 100173399 Orangutan
- Omim: 609505 Human
- SwissProt: O95361 Human
- SwissProt: Q99PP9 Mouse
- SwissProt: Q5R760 Orangutan
- Unigene: 123534 Human
see all -
Alternative names
- EBBP antibody
- Estrogen responsive B box protein antibody
- Estrogen-responsive B box protein antibody
see all
Images
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All lanes : Anti-TRIM16 antibody (ab72129) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Immunoprecipitation/ Western Blot of TRIM16.
Lane 1: ab72129 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72129 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72129 has been referenced in 2 publications.
- Wang Y et al. CircPTK2 inhibits cell cisplatin (CDDP) resistance by targeting miR-942/TRIM16 axis in non-small cell lung cancer (NSCLC). Bioengineered 13:3651-3664 (2022). PubMed: 35230201
- Wang N & Zhang T Downregulation of MicroRNA-135 Promotes Sensitivity of Non-Small Cell Lung Cancer to Gefitinib by Targeting TRIM16. Oncol Res 26:1005-1014 (2018). PubMed: 29295721