Product nameAnti-TRIM16 antibody
See all TRIM16 primary antibodies
DescriptionRabbit polyclonal to TRIM16
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 514 and 564 of human TRIM16, NP_006461.3.
- Whole cell lysate from Hela, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay play a role in the regulation of keratinocyte differentiation.
Tissue specificityHighest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
- Information by UniProt
- EBBP antibody
- Estrogen responsive B box protein antibody
- Estrogen-responsive B box protein antibody
All lanes : Anti-TRIM16 antibody (ab72131) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Various other fainter bands are detected, ranging from 16-250 kDa.
Immunoprecipitation/ Western Blot of TRIM16.
Lane 1: ab72131 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72131 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 3 seconds.
ab72131 has not yet been referenced specifically in any publications.