Anti-TRIM2 antibody (ab3942)
Key features and details
- Goat polyclonal to TRIM2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM2 antibody -
Description
Goat polyclonal to TRIM2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
Sequence:DSGNHCFKVYRYLQ
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 µg/ml.
A 1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. A 1 hour primary incubation is recommended for this product. |
Target
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Function
May contribute to the alteration of neural cellular mechanisms. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger. -
Domain
The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23321 Human
- Entrez Gene: 361970 Rat
- Omim: 614141 Human
- SwissProt: Q9C040 Human
- SwissProt: D3ZQG6 Rat
- Unigene: 435711 Human
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Alternative names
- E3 ubiquitin protein ligase TRIM2 antibody
- KIAA0517 antibody
- RING finger protein 86 antibody
see all
Images
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Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.Primary incubation was for 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab3942 has been referenced in 2 publications.
- Yi H et al. Knockdown of long non-coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA-369-3p/TRIM2 axis. Int J Mol Med 47:N/A (2021). PubMed: 33760118
- Qin Y et al. TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. Int J Oncol 53:1643-1656 (2018). PubMed: 30066883