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Goat polyclonal to TRIM2
Mouse, Rat, HumanPredicted to work with:
Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
- WB: Rat brain tissue lysate.
IHC-P: Human cortex tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 0.5 µg/ml.
||Use at an assay dependent dilution. Peptide ELISA (antibody detection limit dilution 1:16,000).
May contribute to the alteration of neural cellular mechanisms.
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger.
The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Information by UniProt
- E3 ubiquitin protein ligase TRIM2 antibody
- KIAA0517 antibody
- RING finger protein 86 antibody
Western blot - Anti-TRIM2 antibody (ab3942)
Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µgObserved band size:
80,85 kDa (why is the actual band size different from the predicted?
)Additional bands at:
30 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was for 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM2 antibody (ab3942)
ab3942 at 6.3 µg/ml staining TRIM2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"