Key features and details
- Mouse polyclonal to TRIM22
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM22 antibody
See all TRIM22 primary antibodies
DescriptionMouse polyclonal to TRIM22
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human TRIM22 aa 1-498.
- Trim22 transfected mammalian cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).|
RelevanceTrim22 is an interferon inducible protein that is preferentially expressed in cells of the haematopoietic system. Trim22 has been shown to be a p53 target gene. It also has an activation stage specific role connected to the paracrine crosstalk during T lymphocyte activation. It is strongly expressed in peripheral blood leukocytes, spleen, thymus, and ovary; expressed at basal levels in other tissues. There are two named isoforms.
Cellular localizationCytoplasm. Nucleus. Nucleus speckle. Nucleus; Cajal body. Note: Localizes predominanltly into the nucleus, found in cytoplasm to some extent. Forms distinct nuclear bodies that undergo dynamic changes during cell cycle progression. Nuclear bodies start to form in the early G0/G1 phase but become speckle-like in the S-phase and completely dispersed in mitosis. 35% of TRIM22 nuclear bodies overlap or are found adjacent to Cajal bodies.
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All lanes : Anti-TRIM22 antibody (ab68071) at 1/500 dilution
Lane 1 : Trim22 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab68071 has not yet been referenced specifically in any publications.