Recombinant
RabMAb

Anti-TRIM23 antibody [EPR7787] (ab192032)

Overview

  • Product name
    Anti-TRIM23 antibody [EPR7787]
    See all TRIM23 primary antibodies
  • Description
    Rabbit monoclonal [EPR7787] to TRIM23
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TRIM23 aa 450-550. The exact sequence is proprietary.
    Database link: P36406

  • Positive control
    • HeLa, 293T, C6, RAW 264.7, PC.-12 and NIH 3T3 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR7787
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

Target

  • Function
    Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    In the C-terminal section; belongs to the small GTPase superfamily. Arf family.
    Contains 1 B box-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
  • Cellular localization
    Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Membrane-associated with the Golgi complex and lysosomal structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor domain protein 1 antibody
    • ADP-ribosylation factor domain-containing protein 1 antibody
    • ARF domain protein 1 antibody
    • ARFD1 antibody
    • E3 ubiquitin-protein ligase TRIM23 antibody
    • GTP binding protein ARD 1 antibody
    • GTP-binding protein ARD-1 antibody
    • N-acetyltransferase ARD1 human homolog of antibody
    • N-acetyltransferase homolog of S. cerevisiae ARD1 antibody
    • RING finger protein 46 antibody
    • TRI23_HUMAN antibody
    • TRIM23 antibody
    • Tripartite motif protein TRIM23 antibody
    • Tripartite motif-containing protein 23 antibody
    see all

Images

  • All lanes : Anti-TRIM23 antibody [EPR7787] (ab192032) at 1/50000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293T lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa

  • All lanes : Anti-TRIM23 antibody [EPR7787] (ab192032) at 1/10000 dilution

    Lane 1 : C6 lysate
    Lane 2 : RAW 264.7 lysate
    Lane 3 : PC12 lysate
    Lane 4 : NIH 3T3 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa

References

ab192032 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192032.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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