Anti-TRIM23/ARD1 antibody (ab97291)
Key features and details
- Rabbit polyclonal to TRIM23/ARD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM23/ARD1 antibody
See all TRIM23/ARD1 primary antibodies -
Description
Rabbit polyclonal to TRIM23/ARD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TRIM23/ARD1 aa 2-14.
Database link: NP_150230 -
Positive control
- WB: HeLaS3 whole cell lysate IHC-P: Gastric CA ICC/IF: HeLa cell
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General notes
This product was previously labelled as TRIM23
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab97291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/300. Predicted molecular weight: 64 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
In the C-terminal section; belongs to the small GTPase superfamily. Arf family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity. -
Cellular localization
Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Membrane-associated with the Golgi complex and lysosomal structures. - Information by UniProt
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Database links
- Entrez Gene: 373 Human
- Omim: 601747 Human
- SwissProt: P36406 Human
- Unigene: 792 Human
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Alternative names
- ADP ribosylation factor domain protein 1 antibody
- ADP-ribosylation factor domain-containing protein 1 antibody
- ARF domain protein 1 antibody
see all
Images
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Western blot - Anti-TRIM23/ARD1 antibody (ab97291)Anti-TRIM23/ARD1 antibody (ab97291) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 64 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM23/ARD1 antibody (ab97291)Immunohistochemical analysis of TRIM23/ARD1 in paraffin embedded gastric CA, using ab97291 at a 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM23/ARD1 antibody (ab97291)Immunofluorescence analysis of TRIM23/ARD1 in paraformaldehyde fixed HeLa, using ab97291 at a 1/200 dilution.
Image 1: without co-staining; image 2: co-stained with Hoechst 33342.
Protocols
References (2)
ab97291 has been referenced in 2 publications.
- Yao Y et al. Elevated TRIM23 expression predicts poor prognosis in Chinese gastric cancer. Pathol Res Pract 214:2062-2068 (2018). PubMed: 30477642
- Sparrer KMJ et al. TRIM23 mediates virus-induced autophagy via activation of TBK1. Nat Microbiol 2:1543-1557 (2017). WB, IP, ICC/IF ; Human . PubMed: 28871090
