Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7787] to TRIM23/ARD1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-TRIM23/ARD1 antibody [EPR7787] - BSA and Azide free
See all TRIM23/ARD1 primary antibodies
DescriptionRabbit monoclonal [EPR7787] to TRIM23/ARD1 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TRIM23/ARD1 aa 450-550. The exact sequence is proprietary.
Database link: P36406
- WB: HeLa and 293T lysates.
ab239010 is the carrier-free version of ab192032 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab239010 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TRIM23
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab239010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
FunctionActs as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesIn the C-terminal section; belongs to the small GTPase superfamily. Arf family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
Cellular localizationEndomembrane system. Golgi apparatus membrane. Lysosome membrane. Membrane-associated with the Golgi complex and lysosomal structures.
- Information by UniProt
- ADP ribosylation factor domain protein 1 antibody
- ADP-ribosylation factor domain-containing protein 1 antibody
- ARF domain protein 1 antibody
All lanes : Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032) at 1/50000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 64 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192032).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239010 has not yet been referenced specifically in any publications.