Anti-TRIM24 antibody (ab211300)
Key features and details
- Rabbit polyclonal to TRIM24
- Suitable for: WB, ICC/IF, IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRIM24 antibody
See all TRIM24 primary antibodies -
Description
Rabbit polyclonal to TRIM24 -
Host species
Rabbit -
Specificity
At least two isoforms of TRIM24 are known to exist. -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human TRIM24 (internal sequence). 19 amino acid peptide. AAD17258.1.
Database link: O15164-2 -
Positive control
- Human testis tissue; EL4 cells and cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 113 kDa.
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 113 kDa. |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC
Use a concentration of 2.5 µg/ml. |
Target
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Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene. -
Sequence similarities
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 8805 Human
- Entrez Gene: 21848 Mouse
- Entrez Gene: 500084 Rat
- Omim: 603406 Human
- SwissProt: O15164 Human
- SwissProt: Q64127 Mouse
- Unigene: 490287 Human
- Unigene: 41063 Mouse
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Alternative names
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
see all
Images
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Lane 1 : Anti-TRIM24 antibody (ab211300) at 0.5 µg/ml
Lane 2 : Anti-TRIM24 antibody (ab211300) at 1 µg/ml
All lanes : EL4 cell lysate
Predicted band size: 113 kDa -
Immunofluorescent analysis of EL4 (mouse) cells labeling TRIM24 with ab211300 at 20µg/ml.
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Immunocytochemical analysis of EL4 (mouse) cells labelling TRIM24 with ab211300 at 2.5µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling TRIM24 with ab211300 at 10µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab211300 has been referenced in 1 publication.
- Guan Y et al. Epigenetic silencing of miR-137 induces resistance to bicalutamide by targeting TRIM24 in prostate cancer cells. Am J Transl Res 11:3226-3237 (2019). PubMed: 31217891