Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM24
  • Host species

    Rabbit
  • Specificity

    At least two isoforms of TRIM24 are known to exist.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human TRIM24 (internal sequence). 19 amino acid peptide. AAD17258.1.
    Database link: O15164-2

  • Positive control

    • Human testis tissue; EL4 cells and cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab211300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 113 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 2.5 µg/ml.

Target

  • Function

    Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
  • Sequence similarities

    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • Cellular localization

    Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase TRIM24 antibody
    • E3 ubiquitin-protein ligase Trim24 antibody
    • hTIF1 antibody
    • PTC6 antibody
    • RING finger protein 82 antibody
    • RNF82 antibody
    • TF1A antibody
    • TIF1 alpha antibody
    • TIF1 antibody
    • TIF1-alpha antibody
    • TIF1A antibody
    • TIF1A_HUMAN antibody
    • TIF1ALPHA antibody
    • Transcription intermediary factor 1-alpha antibody
    • Transcriptional intermediary factor 1 alpha antibody
    • Transcriptional intermediary factor 1 antibody
    • Trim24 antibody
    • Tripartite motif containing 24 antibody
    • Tripartite motif-containing protein 24 antibody
    see all

Images

  • Lane 1 : Anti-TRIM24 antibody (ab211300) at 0.5 µg/ml
    Lane 2 : Anti-TRIM24 antibody (ab211300) at 1 µg/ml

    All lanes : EL4 cell lysate

    Predicted band size: 113 kDa

  • Immunofluorescent analysis of EL4 cells labeling TRIM24 with ab211300 at 20µg/ml.

  • Immunocytochemical analysis of EL4 cells labelling TRIM24 with ab211300 at 2.5µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling TRIM24 with ab211300 at 10µg/ml.

References

ab211300 has not yet been referenced specifically in any publications.

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