Key features and details
- Rabbit polyclonal to TRIM24
- Suitable for: WB, ICC/IF, IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TRIM24 antibody
See all TRIM24 primary antibodies
DescriptionRabbit polyclonal to TRIM24
SpecificityAt least two isoforms of TRIM24 are known to exist.
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human TRIM24 (internal sequence). 19 amino acid peptide. AAD17258.1.
Database link: O15164-2
- Human testis tissue; EL4 cells and cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 113 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use a concentration of 2.5 µg/ml.|
FunctionTranscriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
Sequence similaritiesContains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
- Information by UniProt
- E3 ubiquitin protein ligase TRIM24 antibody
- E3 ubiquitin-protein ligase Trim24 antibody
- hTIF1 antibody
Lane 1 : Anti-TRIM24 antibody (ab211300) at 0.5 µg/ml
Lane 2 : Anti-TRIM24 antibody (ab211300) at 1 µg/ml
All lanes : EL4 cell lysate
Predicted band size: 113 kDa
Immunofluorescent analysis of EL4 (mouse) cells labeling TRIM24 with ab211300 at 20µg/ml.
Immunocytochemical analysis of EL4 (mouse) cells labelling TRIM24 with ab211300 at 2.5µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling TRIM24 with ab211300 at 10µg/ml.
ab211300 has been referenced in 1 publication.
- Guan Y et al. Epigenetic silencing of miR-137 induces resistance to bicalutamide by targeting TRIM24 in prostate cancer cells. Am J Transl Res 11:3226-3237 (2019). PubMed: 31217891