Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human TRIM24 aa 700-750. The exact sequence is proprietary.
    Database link: NP_056989.2

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab70560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 116 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM24/TIF1 is found in thyroid papillary carcinomas. Translocation t(7;10)(q32;q11) with RET. The translocation generates the TRIM24/RET (PTC6) oncogene.
  • Sequence similarities

    Contains 2 B box-type zinc fingers.
    Contains 1 bromo domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated.
  • Cellular localization

    Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase TRIM24 antibody
    • E3 ubiquitin-protein ligase Trim24 antibody
    • hTIF1 antibody
    • PTC6 antibody
    • RING finger protein 82 antibody
    • RNF82 antibody
    • TF1A antibody
    • TIF1 alpha antibody
    • TIF1 antibody
    • TIF1-alpha antibody
    • TIF1A antibody
    • TIF1A_HUMAN antibody
    • TIF1ALPHA antibody
    • Transcription intermediary factor 1-alpha antibody
    • Transcriptional intermediary factor 1 alpha antibody
    • Transcriptional intermediary factor 1 antibody
    • Trim24 antibody
    • Tripartite motif containing 24 antibody
    • Tripartite motif-containing protein 24 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TRIM24  with ab70560 at 1/1000 (0.2µg/ml). Detection: DAB.

  • IHC image of TRIM24 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70560, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TRIM24 antibody (ab70560) at 0.04 µg/ml

    Lane 1 : HeLa whole cell
    lysate at 50 µg
    Lane 2 : HeLa whole cell
    lysate at 15 µg
    Lane 3 : HeLa whole cell
    lysate at 5 µg
    Lane 4 : 293T whole cell
    lysate at 50 µg
    Lane 5 : NIH3T3whole cell
    lysate at 50 µg

    Predicted band size: 116 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds

References

This product has been referenced in:

  • Fyfe JJ  et al. Enhanced skeletal muscle ribosome biogenesis, yet attenuated mTORC1 and ribosome biogenesis-related signalling, following short-term concurrent versus single-mode resistance training. Sci Rep 8:560 (2018). Read more (PubMed: 29330460) »
  • Fang Z  et al. TRIM24 promotes the aggression of gastric cancer via the Wnt/ß-catenin signaling pathway. Oncol Lett 13:1797-1806 (2017). WB, IHC-P ; Human . Read more (PubMed: 28454326) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
We have 3 antibodies to TIF1 alpha: ab70560, ab57994 and ab84381.
Two of them have been tested in mouse (ab70560 and ab84381), and ab84381 was already tested and would be guaranteed for mouse and human paraffin-embedded IHC samples (IHC-P).
What type of IHC do you have in mind?
We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Specification
Brain
Blocking step
No blocking step used for 30 minute(s) · Concentration: 4%
Fixative
Formaldehyde

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Verified customer

Submitted Aug 07 2015

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