Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM25/EFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIM25/EFP aa 75-125. The exact sequence is proprietary. NP_005073.2
    Database link: Q14258

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as TRIM25

     

Properties

Applications

Our Abpromise guarantee covers the use of ab245486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.

Target

  • Function

    Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • Tissue specificity

    Ubiquitous.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • Post-translational
    modifications

    Auto-ISGylated.
  • Cellular localization

    Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin/ISG15 ligase TRIM25 antibody
    • EFP antibody
    • Estrogen responsive finger protein antibody
    • Estrogen-responsive finger protein antibody
    • RING finger protein 147 antibody
    • RNF 147 antibody
    • RNF147 antibody
    • TRI25 antibody
    • TRI25_HUMAN antibody
    • TRIM 25 antibody
    • Trim25 antibody
    • Tripartite motif containing 25 antibody
    • Tripartite motif containing protein 25 antibody
    • Tripartite motif protein TRIM25 antibody
    • Tripartite motif-containing protein 25 antibody
    • Tripartite motifcontaining25 antibody
    • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
    • Z147 antibody
    • Zinc finger protein 147 (estrogen responsive finger protein) antibody
    • Zinc finger protein 147 antibody
    • Zinc finger protein-147 antibody
    • ZNF 147 antibody
    • ZNF147 antibody
    see all

Images

  • All lanes : Anti-TRIM25/EFP antibody (ab245486) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 71 kDa


    Exposure time: 10 seconds
  • TRIM25/EFP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245486 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245486 at 1 µg/ml.

    Lane 1: ab245486 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

References

ab245486 has not yet been referenced specifically in any publications.

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