Key features and details
- Rabbit polyclonal to TRIM25/EFP
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM25/EFP antibody
See all TRIM25/EFP primary antibodies
DescriptionRabbit polyclonal to TRIM25/EFP
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TRIM25/EFP aa 75-125. The exact sequence is proprietary. NP_005073.2
Database link: Q14258
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as TRIM25
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245486 was affinity purified using an epitope specific to TRIM25/EFP immobilized on solid support.
Our Abpromise guarantee covers the use of ab245486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
FunctionFunctions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
Cellular localizationCytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
- Information by UniProt
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
All lanes : Anti-TRIM25/EFP antibody (ab245486) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 10 seconds
TRIM25/EFP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245486 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245486 at 1 µg/ml.
Lane 1: ab245486 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245486 has not yet been referenced specifically in any publications.