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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase
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Anti-TRIM25/EFP antibody (ab245487)

  • Datasheet
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Western blot - Anti-TRIM25/EFP antibody (ab245487)
  • Immunoprecipitation - Anti-TRIM25/EFP antibody (ab245487)

Key features and details

  • Rabbit polyclonal to TRIM25/EFP
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TRIM25/EFP protein (ab152814)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TRIM25/EFP antibody
    See all TRIM25/EFP primary antibodies
  • Description

    Rabbit polyclonal to TRIM25/EFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TRIM25/EFP aa 375-425. The exact sequence is proprietary. NP_005073.2
    Database link: Q14258

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as TRIM25

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245487 was affinity purified using an epitope specific to TRIM25/EFP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human TRIM25/EFP protein (ab152814)
  • Related Products

    • Recombinant Human TRIM25/EFP protein (ab152814)

Applications

Our Abpromise guarantee covers the use of ab245487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.

Target

  • Function

    Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
  • Tissue specificity

    Ubiquitous.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
    The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
  • Post-translational
    modifications

    Auto-ISGylated.
  • Cellular localization

    Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
  • Target information above from: UniProt accession Q14258 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7706 Human
    • Omim: 600453 Human
    • SwissProt: Q14258 Human
    • Unigene: 528952 Human
    • Alternative names

      • E3 ubiquitin/ISG15 ligase TRIM25 antibody
      • EFP antibody
      • Estrogen responsive finger protein antibody
      • Estrogen-responsive finger protein antibody
      • RING finger protein 147 antibody
      • RNF 147 antibody
      • RNF147 antibody
      • TRI25 antibody
      • TRI25_HUMAN antibody
      • TRIM 25 antibody
      • Trim25 antibody
      • Tripartite motif containing 25 antibody
      • Tripartite motif containing protein 25 antibody
      • Tripartite motif protein TRIM25 antibody
      • Tripartite motif-containing protein 25 antibody
      • Tripartite motifcontaining25 antibody
      • Ubiquitin/ISG15-conjugating enzyme TRIM25 antibody
      • Z147 antibody
      • Zinc finger protein 147 (estrogen responsive finger protein) antibody
      • Zinc finger protein 147 antibody
      • Zinc finger protein-147 antibody
      • ZNF 147 antibody
      • ZNF147 antibody
      see all

    Images

    • Western blot - Anti-TRIM25/EFP antibody (ab245487)
      Western blot - Anti-TRIM25/EFP antibody (ab245487)
      All lanes : Anti-TRIM25/EFP antibody (ab245487) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 71 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-TRIM25/EFP antibody (ab245487)
      Immunoprecipitation - Anti-TRIM25/EFP antibody (ab245487)

      TRIM25/EFP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245487 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245487 at 1 µg/ml.

      Lane 1: ab245487 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245487? Please let us know so that we can cite the reference in this datasheet.

    ab245487 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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