Product nameAnti-TRIM25/EFP antibody
See all TRIM25/EFP primary antibodies
DescriptionRabbit polyclonal to TRIM25/EFP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide corresponding to Human TRIM25/EFP aa 325-375.
Database link: NP_005073.2
- HeLa or 293T cell lysate.
This product was previously labelled as TRIM25
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 71 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionFunctions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation negatively regulates ISG15 E3 ligase activity.
The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58.
Cellular localizationCytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.
- Information by UniProt
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
All lanes : Anti-TRIM25/EFP antibody (ab86365) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 71 kDa
Exposure time: 30 seconds
Detection of TRIM25/EFP by Western Blot of Immunprecipitate.
ab86365 at 1µg/ml staining TRIM25/EFP in HeLa whole cell lysate immunoprecipitated using ab86365 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
This product has been referenced in:
- Booy EP et al. Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Nucleic Acids Res 46:11575-11591 (2018). Read more (PubMed: 30247708) »
- Hu J et al. A common polymorphism in the caspase recruitment domain of RIG-I modifies the innate immune response of human dendritic cells. J Immunol 185:424-32 (2010). WB ; Human . Read more (PubMed: 20511549) »