Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 213 and 513 of Human TRIM27 (Uniprot ID P14373).

  • Positive control

    • HeLa whole cells lysate; HeLa cells; SW480 xenograft.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.

Target

  • Function

    Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA (By similarity). E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation.
  • Tissue specificity

    Expressed in testis namely within the seminiferous tubules.
  • Involvement in disease

    Defects in TRIM27 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM27/RFP is found in thyroid papillary carcinomas. Translocation t(6;10)(p21.3;q11.2) with RET. The translocation generates TRIM27/RET and delta TRIM27/RET oncogenes.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus > PML body. Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW538890 antibody
    • DAAP-182E11.3 antibody
    • MGC189472 antibody
    • OTTHUMP00000029314 antibody
    • Ret finger protein antibody
    • RFP antibody
    • RFP transforming protein antibody
    • Rfp1 antibody
    • RING finger protein 76 antibody
    • RNF76 antibody
    • TRI27_HUMAN antibody
    • TRIM 27 antibody
    • Trim27 antibody
    • Tripartite motif containing 27 antibody
    • Tripartite motif containing protein 27 antibody
    • Tripartite motif protein TRIM27 antibody
    • Tripartite motif-containing protein 27 antibody
    • Zinc finger protein RFP antibody
    see all

Images

  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling ab137368 using ab137638 (Green) at 1/500 dilution. Alpha-tubulin filaments were labelled in Red.
  • Anti-TRIM27 antibody (ab137638) at 1/1000 dilution + HeLa whole cells lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS Page
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft, labelling TRIM27 using ab137638 antibody at a 1/100 dilution.

References

ab137638 has not yet been referenced specifically in any publications.

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