Key features and details
- Rabbit polyclonal to TRIM29 (acetyl K116)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM29 (acetyl K116) antibody
See all TRIM29 primary antibodies
DescriptionRabbit polyclonal to TRIM29 (acetyl K116)
SpecificityReacts with over-expressed, acetylated lysine TRIM29. Cross-reactivity with TRIM29 from other sources has not been determined.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee, Macaque monkey
Synthetic peptide corresponding to an internal portion of Human TRIM29 around lysine 116.
- 293T cells transfected with acetylated (K116) TRIM29
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
FunctionIt is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line.
Tissue specificityExpressed in placenta, prostate and thymus.
Sequence similaritiesContains 1 B box-type zinc finger.
modificationsConstitutively phosphorylated by PKC on serine/threonine in A431 cells.
Cellular localizationCytoplasm. Colocalizes with intermediate filaments.
- Information by UniProt
- Ataxia telangiectasia group D associated protein antibody
- Ataxia telangiectasia group D-associated protein antibody
- ATDC antibody
All lanes : Anti-TRIM29 (acetyl K116) antibody (ab106001) at 1/500 dilution
Lane 1 : Empty Vector
Lane 2 : Empty Vector plus HDAC inhibitor (TSA)
Lane 3 : TRIM29 WT
Lane 4 : TRIM29 WT plus HDAC inhibitor (TSA)
Lane 5 : TRIM29 K115R
Lane 6 : TRIM29 K115R plus HDAC inhibitor (TSA)
Predicted band size: 66 kDa
Western blot shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) TRIM29 (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an TRIM29 K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3 µM, 6 hr) which inhibits deacetylation.
ab106001 has not yet been referenced specifically in any publications.