Recombinant Anti-TRIM29 antibody [EPR3494] (ab108627)
![Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-389685-anti-trim29-antibody-epr3494-western-blot-human-lysate.jpg "Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3494] to TRIM29
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRIM29 antibody [EPR3494]
See all TRIM29 primary antibodies -
Description
Rabbit monoclonal [EPR3494] to TRIM29 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A431, Daudi, 293T, LnCaP, and JAR cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3494 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa. |
Target
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Function
It is able to complement the radiosensitivity defect of an ataxia telangiectasia (AT) fibroblast cell line. -
Tissue specificity
Expressed in placenta, prostate and thymus. -
Sequence similarities
Contains 1 B box-type zinc finger. -
Post-translational
modificationsConstitutively phosphorylated by PKC on serine/threonine in A431 cells. -
Cellular localization
Cytoplasm. Colocalizes with intermediate filaments. - Information by UniProt
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Database links
- Entrez Gene: 23650 Human
- Entrez Gene: 72169 Mouse
- Entrez Gene: 300656 Rat
- Omim: 610658 Human
- SwissProt: Q14134 Human
- SwissProt: Q8R2Q0 Mouse
- Unigene: 504115 Human
- Unigene: 273277 Mouse
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Alternative names
- Ataxia telangiectasia group D associated protein antibody
- Ataxia telangiectasia group D-associated protein antibody
- ATDC antibody
see all
Images
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Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRIM29 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 66 kDaLanes 1-4: Merged signal (red and green). Green - ab108627 observed at 74 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108627 Anti-TRIM29 antibody [EPR3494] was shown to specifically react with TRIM29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265878 (knockout cell lysate ab257770) was used. Wild-type and TRIM29 knockout samples were subjected to SDS-PAGE. ab108627 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-121378-anti-trim29-antibody-epr3494-western-blot.jpg)
Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
![Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-3-benefits-of-recombinant-antibodies.png)
Anti-TRIM29 antibody [EPR3494] (ab108627)
Datasheets and documents
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Datasheet download
References (4)
ab108627 has been referenced in 4 publications.
- Liu G et al. ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3. J Cell Mol Med 25:3524-3536 (2021). PubMed: 33683826
- Lei G et al. TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. Can J Gastroenterol Hepatol 2021:8870907 (2021). PubMed: 33824865
- Yang Y et al. MicroRNA-122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in vitro. Mol Med Rep 17:8244-8252 (2018). PubMed: 29693120
- Xu W et al. RNA interference against TRIM29 inhibits migration and invasion of colorectal cancer cells. Oncol Rep 36:1411-8 (2016). PubMed: 27430345
