Key features and details
- Rabbit polyclonal to TRIM3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM3 antibody
DescriptionRabbit polyclonal to TRIM3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide from N terminal residues of Human TRIM3 protein (NP_006449).
- Human fetal heart, Human fetal brain, 293T, RAW264.7 and LoVo lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 200ul Sterile Distilled Water.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111840 is purified by a peptide affinity column.
Our Abpromise guarantee covers the use of ab111840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 81 kDa.|
RelevanceTRIM3 a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM3 localizes to cytoplasmic filaments and is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.
Cellular localizationCytoplasmic; Early endosome
- BERP antibody
- Brain expressed RING finger protein antibody
- FLJ16135 antibody
All lanes : Anti-TRIM3 antibody (ab111840) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : 293T lysate
Lane 4 : RAW264.7 lysate
Lane 5 : LoVo lysate
Observed band size: 81 kDa why is the actual band size different from the predicted?
ab111840 has been referenced in 3 publications.
- Song Y et al. miR-454-3p promotes proliferation and induces apoptosis in human cervical cancer cells by targeting TRIM3. Biochem Biophys Res Commun 516:872-879 (2019). PubMed: 31270028
- Li Y et al. miR-4513 promotes breast cancer progression through targeting TRIM3. Am J Transl Res 11:2431-2438 (2019). PubMed: 31105849
- Lu Q et al. TRIM3 Negatively Regulates Autophagy Through Promoting Degradation of Beclin1 in Ewing Sarcoma Cells. Onco Targets Ther 12:11587-11595 (2019). PubMed: 32021240