Overview

  • Product name

  • Description

    Rabbit polyclonal to TRIM30/Rpt1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse TRIM30/Rpt1 (N terminal).
    Database link: NP_033125

  • Positive control

    • Mouse heart tissue lysate.
  • General notes

     This product was previously labelled as TRIM30

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 24756037

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Trans acting factor that regulates gene expression of interleukin 2 receptor alpha chain and of human immunodeficiency virus type 1. May affect IL2R alpha and HIV 1 expression through cis acting negative regulatory elements or through competition with proteins that bind to enhancer or activator sequences.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Down regulatory protein of interleukin 2 receptor antibody
    • Rpt 1 antibody
    • Rpt1 antibody
    • Trim 30 antibody
    • Tripartite motif containing 30 antibody
    see all

Images

  • Lane 1 : Anti-TRIM30/Rpt1 antibody (ab76953) at 1 µg/ml
    Lane 2 : Anti-TRIM30/Rpt1 antibody (ab76953) at 2 µg/ml

    All lanes : Mouse heart tissue lysate

    Predicted band size: 57 kDa

  • ab76953 at 10 µg/ml staining TRIM30/Rpt1 in mouse ovary tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of TRIM30/Rpt1 in Human Ovary cells using ab76953 at 20 ug/ml.

References

This product has been referenced in:

  • Choi UY  et al. Tripartite Motif-Containing Protein 30 Modulates TCR-Activated Proliferation and Effector Functions in CD4+ T Cells. PLoS One 9:e95805 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24756037) »
See 1 Publication for this product

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