• Product name
  • Description
    Rabbit polyclonal to TRIM32
  • Host species
  • Specificity
    This antibody reacts with TRIM32
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MAAAAASHLN LDALREVLEC PICMESFTEE QLRPKLLHCG HTICRQCLEK, corresponding to N terminal amino acids 1-50 of Human TRIM32

  • Positive control
    • Jurkat whole cell lysate (ab7899)



Our Abpromise guarantee covers the use of ab50555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.


  • Function
    Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
  • Tissue specificity
    Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 5 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Post-translational
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kda Tat interacting Protein antibody
    • 72 kDa Tat-interacting protein antibody
    • BBS11 antibody
    • E3 ubiquitin-protein ligase TRIM32 antibody
    • HT2A antibody
    • LGMD2H antibody
    • Limb girdle muscular dystrophy 2H (autosomal recessive) antibody
    • Limb girdle muscular dystrophy 2H antibody
    • Muscular dystrophy Hutterite type antibody
    • TAT interactive protein 72KD antibody
    • TATIP antibody
    • TRI32_HUMAN antibody
    • Trim32 antibody
    • Tripartite Motif Containing Protein 32 antibody
    • Tripartite motif-containing protein 32 antibody
    • Zinc finger protein HT2A antibody
    see all


  • Lane 2 : Anti-TRIM32 antibody (ab50555) at 1.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : Jurkat cell lysate at 10 µg

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 72 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


This product has been referenced in:
  • Streich FC  et al. Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism. J Biol Chem 288:8209-21 (2013). WB ; Human . Read more (PubMed: 23408431) »
See 1 Publication for this product

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