• Product name
  • Description
    Rabbit polyclonal to TRIM32
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1-259 of Human TRIM32 (AAH03154).

  • Positive control
    • 293T and MOLT4 whole cell lysates; H441 Xenograft.



Our Abpromise guarantee covers the use of ab96612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/500.


  • Function
    Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
  • Tissue specificity
    Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 5 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Post-translational
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kda Tat interacting Protein antibody
    • 72 kDa Tat-interacting protein antibody
    • BBS11 antibody
    • E3 ubiquitin-protein ligase TRIM32 antibody
    • HT2A antibody
    • LGMD2H antibody
    • Limb girdle muscular dystrophy 2H (autosomal recessive) antibody
    • Limb girdle muscular dystrophy 2H antibody
    • Muscular dystrophy Hutterite type antibody
    • TAT interactive protein 72KD antibody
    • TATIP antibody
    • TRI32_HUMAN antibody
    • Trim32 antibody
    • Tripartite Motif Containing Protein 32 antibody
    • Tripartite motif-containing protein 32 antibody
    • Zinc finger protein HT2A antibody
    see all


  • Anti-TRIM32 antibody (ab96612) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 72 kDa

    7.5% SDS PAGE.
  • ab96612 at 1/100 dilution staining TRIM32 in H441 Xenograft by Immunohistochemistry, Paraffin-embedded tissue.
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using TRIM32(ab96612) antibody at 1:500 dilution.


This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your inquiry.

Unfortunately we do not have any publications for this antibody yet. Also, we do not have any trial sized samples as we only have the sizes in stock that we sell (100ul).

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Thank you for contacting us.
You are correct, the WB image show TRIM32 72 KDA band in 293T cell lysates.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


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