Anti-TRIM32 antibody (ab96612)

Submit a review Q&A (3)References (6)
Western blot - Anti-TRIM32 antibody (ab96612)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM32 antibody (ab96612)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM32 antibody (ab96612)

Key features and details

  • Rabbit polyclonal to TRIM32
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-TRIM32 antibody
    See all TRIM32 primary antibodies

  • Description

    Rabbit polyclonal to TRIM32

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1-259 of Human TRIM32 (AAH03154).

  • Positive control

    • 293T and MOLT4 whole cell lysates; H441 Xenograft.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab96612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

  • Tissue specificity

    Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

  • Pathway

    Protein modification; protein ubiquitination.

  • Involvement in disease

    Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.

  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 5 NHL repeats.
    Contains 1 RING-type zinc finger.

  • Post-translational
    modifications

    Ubiquitinated.

  • Cellular localization

    Cytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 72 kda Tat interacting Protein antibody
    • 72 kDa Tat-interacting protein antibody
    • BBS11 antibody
    • E3 ubiquitin-protein ligase TRIM32 antibody
    • HT2A antibody
    • LGMD2H antibody
    • Limb girdle muscular dystrophy 2H (autosomal recessive) antibody
    • Limb girdle muscular dystrophy 2H antibody
    • Muscular dystrophy Hutterite type antibody
    • TAT interactive protein 72KD antibody
    • TATIP antibody
    • TRI32_HUMAN antibody
    • Trim32 antibody
    • Tripartite Motif Containing Protein 32 antibody
    • Tripartite motif-containing protein 32 antibody
    • Zinc finger protein HT2A antibody
    see all

Images

  • Western blot - Anti-TRIM32 antibody (ab96612)
    Western blot - Anti-TRIM32 antibody (ab96612)
    Anti-TRIM32 antibody (ab96612) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    7.5% SDS PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM32 antibody (ab96612)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM32 antibody (ab96612)
    ab96612 at 1/100 dilution staining TRIM32 in H441 Xenograft by Immunohistochemistry, Paraffin-embedded tissue.
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM32 antibody (ab96612)
    Immunocytochemistry/ Immunofluorescence - Anti-TRIM32 antibody (ab96612)
    Immunofluorescence analysis of paraformaldehyde-fixed A431, using TRIM32(ab96612) antibody at 1:500 dilution.

References (6)

Publishing research using ab96612? Please let us know so that we can cite the reference in this datasheet.

ab96612 has been referenced in 6 publications.

  • Luo Q  et al. TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma. Cell Rep 30:98-111.e5 (2020). PubMed: 31914402
  • Lazzari E  et al. Analysis of the Zn-Binding Domains of TRIM32, the E3 Ubiquitin Ligase Mutated in Limb Girdle Muscular Dystrophy 2H. Cells 8:N/A (2019). PubMed: 30884854
  • Wilczynska A  et al. eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR. Genome Biol 20:262 (2019). PubMed: 31791371
  • Ito M  et al. Overexpression of E3 ubiquitin ligase tripartite motif 32 correlates with a poor prognosis in patients with gastric cancer. Oncol Lett 13:3131-3138 (2017). PubMed: 28521418
  • Ruelas DS  et al. MicroRNA-155 Reinforces HIV Latency. J Biol Chem 290:13736-48 (2015). PubMed: 25873391
  • Crawford LJ  et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis 3:e90 (2014). WB ; Human . PubMed: 24590311

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I now have seen that you have a new anti-TRIM32 antibody (ab96612). So far we only used or own costum made anti-TRIM32 antibody, but since the Western-Blot and the IF-staining on the datasheet for your antibody look promising we would be willing to test this antibody. Is it possible to receive some of this antibody for a test to compare it our costum made antibodies? In return we would be willing to provide some pictures and comments.

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Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.


In case of ab96612, tested applications are: WB, IHC-P, ICC/IF (i.e. with cultured cells), and the tested species are human. All these applications/species wold be guaranteed.

The predicted species: Mouse, Rat, Cow are not covered under the guarantee as we do not have data, but only a prediction based on sequence homology.

If the product is to be used in an UNTESTED species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Here are more details:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Question

Are there any peer-reviewed publications that use your Trim32 antibody ab96612? If so, please forward me the citations.

Also, do you have any free sample sizes of this antibody available that we could use to test for our purposes? Thank you.

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Answer

Thank you for your inquiry.


Unfortunately we do not have any publications for this antibody yet. Also, we do not have any trial sized samples as we only have the sizes in stock that we sell (100ul).


How would you like to test this antibody? If you are planning on using it in an untested species or application, you may qualify for our testing discout program (essentially buy one get one free when you submit your data to us).


Please let me know if you would be interested. I look forward to your reply.

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Question

I have an querey about Anti-TRIM32 antibody (ab96612) Are you detecing endogenous Trim32 of 293T lysates using this antibody as mentioned in the datasheet? Thank you

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Answer

Thank you for contacting us.
You are correct, the WB image show TRIM32 72 KDA band in 293T cell lysates.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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