Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6355] to TRIM32
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-TRIM32 antibody [EP6355]
See all TRIM32 primary antibodies
DescriptionRabbit monoclonal [EP6355] to TRIM32
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TRIM32 aa 200-300. The exact sequence is proprietary.
- Human fetal muscle lysate.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.60 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).|
FunctionHas an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Tissue specificitySpleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseLimb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 5 NHL repeats.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
- Information by UniProt
- 72 kda Tat interacting Protein antibody
- 72 kDa Tat-interacting protein antibody
- BBS11 antibody
Anti-TRIM32 antibody [EP6355] (ab131223) at 1/1000 dilution + Human fetal muscle lysate at 10 µg
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 72 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131223 has not yet been referenced specifically in any publications.