Overview

  • Product name

    Anti-TRIM32 antibody [EP6355]
    See all TRIM32 primary antibodies
  • Description

    Rabbit monoclonal [EP6355] to TRIM32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRIM32 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Human fetal muscle lysate.
  • General notes

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
    • Tissue specificity

      Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Limb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
      Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 5 NHL repeats.
      Contains 1 RING-type zinc finger.
    • Post-translational
      modifications

      Ubiquitinated.
    • Cellular localization

      Cytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 72 kda Tat interacting Protein antibody
      • 72 kDa Tat-interacting protein antibody
      • BBS11 antibody
      • E3 ubiquitin-protein ligase TRIM32 antibody
      • HT2A antibody
      • LGMD2H antibody
      • Limb girdle muscular dystrophy 2H (autosomal recessive) antibody
      • Limb girdle muscular dystrophy 2H antibody
      • Muscular dystrophy Hutterite type antibody
      • TAT interactive protein 72KD antibody
      • TATIP antibody
      • TRI32_HUMAN antibody
      • Trim32 antibody
      • Tripartite Motif Containing Protein 32 antibody
      • Tripartite motif-containing protein 32 antibody
      • Zinc finger protein HT2A antibody
      see all

    Images

    • Anti-TRIM32 antibody [EP6355] (ab131223) at 1/1000 dilution + Human fetal muscle lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 72 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131223 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131223.
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