Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6355] to TRIM32 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-TRIM32 antibody [EP6355] - BSA and Azide free
See all TRIM32 primary antibodies
DescriptionRabbit monoclonal [EP6355] to TRIM32 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TRIM32 aa 200-300. The exact sequence is proprietary.
ab248382 is the carrier-free version of ab131223 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 5.60 x 10 -10 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab248382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 78 kDa (predicted molecular weight: 72 kDa).|
FunctionHas an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Tissue specificitySpleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseLimb-girdle muscular dystrophy 2H (LGMD2H) [MIM:254110]: An autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
Bardet-Biedl syndrome 11 (BBS11) [MIM:209900]: A syndrome characterized by usually severe pigmentary retinopathy, early-onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. Bardet-Biedl syndrome inheritance is autosomal recessive, but three mutated alleles (two at one locus, and a third at a second locus) may be required for clinical manifestation of some forms of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 5 NHL repeats.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Localized in cytoplasmic bodies, often located around the nucleus.
- Information by UniProt
- 72 kda Tat interacting Protein antibody
- 72 kDa Tat-interacting protein antibody
- BBS11 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248382 has not yet been referenced specifically in any publications.