Product nameAnti-TRIM33 antibody
See all TRIM33 primary antibodies
DescriptionRabbit polyclonal to TRIM33
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Mouse, Human
Predicted to work with: Xenopus laevis
Synthetic peptide within Human TRIM33 aa 650-700. The exact sequence is proprietary.
Database link: Q9UPN9
- IP: HeLa whole cell lysate; IHC-P: Human osteosarcoma and mouse plasmacytoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264175 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionActs as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
Tissue specificityExpressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseDefects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger.
Cellular localizationNucleus. In discrete nuclear dots resembling nuclear bodies.
- Information by UniProt
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TRIM33 was immunoprecipitated from HeLa cells (1 mg per IP reaction; 20% of IP loaded) using ab264175 at 6 µg per reaction. Western blot was performed on the immunprecipitate using a previous lot of ab264175 at 1 μg/ml.
Lane 1: ab264175 in HeLa whole cell lysate
Lane 2: Control IgG in HeLa whole cell lysate
Detected with chemilimuniscence with exposure time of 10 seconds.
Immunohistochemical analysis of mouse plasmacytoma tissue labeling TRIM33 with ab264175 at 1/1000 dilution. DAB was used as a counterstain.
Immunohistochemical analysis of human osteosarcoma tissue labeling TRIM33 with ab264175 at 1/1000 dilution. DAB was used as a counterstain.
ab264175 has not yet been referenced specifically in any publications.