Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM36
  • Host species
    Rabbit
  • Specificity
    ab171732 recognizes Isoform 1of Human TRIM36.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRIM36 aa 309-339 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9NQ86

  • Positive control
    • Human pancreas tissue, 293 cell line lysate and mouse bladder tissue lysate.
  • General notes

    Previously labelled as RNF98.

Properties

Applications

Our Abpromise guarantee covers the use of ab171732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 83 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
  • Tissue specificity
    Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome RBCC protein antibody
    • E3 ubiquitin protein ligase TRIM36 antibody
    • E3 ubiquitin-protein ligase Trim36 antibody
    • Haprin antibody
    • RBCC728 antibody
    • RING finger protein 98 antibody
    • RNF98 antibody
    • TRI36_HUMAN antibody
    • TRIM 36 antibody
    • Trim36 antibody
    • Tripartite motif containing 36 antibody
    • Tripartite motif containing protein 36 antibody
    • Tripartite motif protein 36 antibody
    • Tripartite motif-containing protein 36 antibody
    • Zinc binding protein Rbcc728 antibody
    • Zinc-binding protein Rbcc728 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling TRIM36 using ab171732 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-TRIM36 antibody (ab171732) at 1/100 dilution + Mouse bladder tissue lysate at 35 µg

    Predicted band size: 83 kDa

  • Anti-TRIM36 antibody (ab171732) at 1/100 dilution + 293 cell line lysate at 35 µg

    Predicted band size: 83 kDa

References

ab171732 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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