Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272672 in the following tested applications.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
Tissue specificityHighly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
- Information by UniProt
- Acrosome RBCC protein antibody
- E3 ubiquitin protein ligase TRIM36 antibody
- E3 ubiquitin-protein ligase Trim36 antibody
All lanes : Anti-TRIM36 antibody (ab272672) at 0.4 µg/ml
Lane 1 : RT4 cells (Human urinary bladder cancer cell line)
Lane 2 : U-251 MG cells (Human brain glioma cell line)
PFA fixed, Triton X-100 permeablized RH-30 cells (human bone marrow metastasis cell line) labeling TRIM36 (green) using ab272672 at 2 ug/ml in ICC/IF analysis.
ab272672 has not yet been referenced specifically in any publications.