Anti-TRIM37/MUL antibody (ab264190)
Key features and details
- Rabbit polyclonal to TRIM37/MUL
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM37/MUL antibody
See all TRIM37/MUL primary antibodies -
Description
Rabbit polyclonal to TRIM37/MUL -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TRIM37/MUL aa 914-964. The exact sequence is proprietary.
Database link: O94972 -
Positive control
- WB: HeLa and HEK-293T cell lysate. IP: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use a concentration of 2 - 5 µg/ml.
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WB |
1/2000 - 1/10000.
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Notes |
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IP
Use a concentration of 2 - 5 µg/ml. |
WB
1/2000 - 1/10000. |
Target
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Function
E3 ubiquitin-protein ligase. -
Tissue specificity
Ubiquitous. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in TRIM37 are the cause of mulibrey nanism (MUL) [MIM:253250]; also known as muscle-liver-brain-eye nanism. MUL is an autosomal recessive disorder that involves several tissues of mesodermal origin, implying a defect in a highly pleiotropic gene. Characteristic features include severe growth failure of prenatal onset and constrictive pericardium with consequent hepatomegaly. In addition, muscle hypotonia, J-shaped sella turcica, yellowish dots in the ocular fundi, typical dysmorphic features and hypoplasia of various endocrine glands causing hormonal deficiency are common. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsAuto-ubiquitinated. -
Cellular localization
Cytoplasm > perinuclear region. Peroxisome. Found in vesicles of the peroxisome. Aggregates as aggresomes, a perinuclear region where certain misfolded or aggregated proteins are sequestered for proteasomal degradation. - Information by UniProt
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Database links
- Entrez Gene: 4591 Human
- Omim: 605073 Human
- SwissProt: O94972 Human
- Unigene: 579079 Human
- Unigene: 605697 Human
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Alternative names
- E3 ubiquitin protein ligase TRIM37 antibody
- E3 ubiquitin-protein ligase TRIM37 antibody
- KIAA0898 antibody
see all
Images
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TRIM37/MUL was immunoprecipitated from 1 mg HeLa whole cell lysate using ab2641890 at 3 µg/mg lysate. ab264190 was then used for western blotting at 1 µg/ml.
Lane 1: ab264189 IP in HeLa whole cell lysate.
Lane 2: ab264190 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Detection: Chemiluminescence with an exposure time of 1 second.
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All lanes : Anti-TRIM37/MUL antibody (ab264190) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab264190 has been referenced in 1 publication.
- Xie H et al. ROS/NF-?B Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis. Mol Ther Nucleic Acids 22:114-123 (2020). PubMed: 32916597