Overview

  • Product name
    Anti-TRIM38 antibody - C-terminal
  • Description
    Rabbit polyclonal to TRIM38 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human TRIM38 aa 422-451 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O00635

  • Positive control
    • HeLa cell lysate, Human colon tissue, HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171850 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • Relevance
    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.
  • Database links
  • Alternative names
    • Gm23 antibody
    • MGC8946 antibody
    • RING finger protein 15 antibody
    • RNF15 antibody
    • Ro/SSA ribonucleoprotein homolog antibody
    • RORET antibody
    • RP23 480B19.4 antibody
    • tripartite motif containing 38 antibody
    • Zinc finger protein RoRet antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin-embedded Human colon tissue, labeling TRIM38 with ab171850  at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-TRIM38 antibody - C-terminal (ab171850) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size: 53 kDa

  • Flow cytometric analysis of Hela cells (right histogram; green) compared to a negative control cell (left histogram; blue), labeling TRIM38 with ab171850 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Kim K  et al. TRIM38 regulates NF-?B activation through TAB2 degradation in osteoclast and osteoblast differentiation. Bone 113:17-28 (2018). Read more (PubMed: 29753717) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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