Key features and details
- Goat polyclonal to TRIM4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM4 antibody
See all TRIM4 primary antibodies
DescriptionGoat polyclonal to TRIM4
SpecificityThis antibody is expected to recognise both human isoforms.
Tested Applications & Species
Application Species WBHuman
General notesGenBank Accession Number – NP_148977; NP_149082.
Previously labelled as RNF87.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa according to NP_148977 and NP_149082).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- RING finger protein 87 antibody
- RNF 87 antibody
- RNF87 antibody
ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
ab4527 has been referenced in 1 publication.
- Feyeux M et al. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Hum Mol Genet 21:3883-95 (2012). PubMed: 22678061