Key features and details
- Rabbit polyclonal to TRIM4 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TRIM4 antibody - N-terminal
See all TRIM4 primary antibodies
DescriptionRabbit polyclonal to TRIM4 - N-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
- WB: MCF7, HeLa and A459 whole cell lysates; Rat brain and heart tissue lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
Previously labelled as RNF87.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229952 in the following tested applications.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 54,62,34,90 kDa (predicted molecular weight: 57,54,34 kDa).|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- RING finger protein 87 antibody
- RNF 87 antibody
- RNF87 antibody
Lanes 1-4 : Anti-TRIM4 antibody - N-terminal (ab229952) at 1/500 dilution
Lane 5 : Anti-TRIM4 antibody - N-terminal (ab229952) at 500 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
All lanes : Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 57,54,34 kDa
Observed band size: 35,55,62,90 kDa why is the actual band size different from the predicted?
Paraffin-embedded human colon cancer tissue stained for TRIM4 using ab229952 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TRIM4 (green) using ab229952 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L) as secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229952 has not yet been referenced specifically in any publications.