Product nameAnti-TRIM4 antibody - N-terminal
See all TRIM4 primary antibodies
DescriptionRabbit polyclonal to TRIM4 - N-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
- WB: MCF7, HeLa and A459 whole cell lysates; Rat brain and heart tissue lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
Previously labelled as RNF87.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 54,62,34,90 kDa (predicted molecular weight: 57,54,34 kDa).|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- RING finger protein 87 antibody
- RNF 87 antibody
- RNF87 antibody
Lanes 1-4 : Anti-TRIM4 antibody - N-terminal (ab229952) at 1/500 dilution
Lane 5 : Anti-TRIM4 antibody - N-terminal (ab229952) at 500 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
All lanes : Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 57,54,34 kDa
Observed band size: 35,55,62,90 kDa why is the actual band size different from the predicted?
Paraffin-embedded human colon cancer tissue stained for TRIM4 using ab229952 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TRIM4 (green) using ab229952 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L) as secondary antibody.
ab229952 has not yet been referenced specifically in any publications.