Overview

  • Product name
  • Description
    Rabbit polyclonal to TRIM47
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 356-638 of Human TRIM47 (Uniprot ID: Q96LD4).

  • Positive control
    • A549 and HeLa whole cell lysate (ab150035); HeLa cells; colon cancer tisue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells.
  • Cellular localization
    Cytoplasm. Nucleus
  • Database links
  • Alternative names
    • Gene overexpressed in astrocytoma protein antibody
    • GOA antibody
    • RING finger protein 100 antibody
    • RNF100 antibody
    • Tripartite motif containing 47 antibody
    • Tripartite motif containing protein 47 antibody
    see all

Images

  • Anti-TRIM47 antibody (ab155549) at 1/2000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TRIM47 with ab155549 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled with an alpha-tubulin monoclonal antibody at 1/2000 dilution (Red).
  • Immunohistochemical analysis of paraffin-embedded colon cancer tissue labeling TRIM47 with ab155549 at 1/250 dilution.

References

ab155549 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Mouse Cell lysate - whole cell (cos-7)
Specification
cos-7
Treatment
transfected with murine trim47-FLAG
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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