1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
1/100 - 1/1000.
TRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TRIM47 with ab155549 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled with an alpha-tubulin monoclonal antibody at 1/2000 dilution (Red).