Key features and details
- Rabbit polyclonal to TRIM47
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM47 antibody
DescriptionRabbit polyclonal to TRIM47
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide from a region between residue 125 and 175 of human TRIM47, NP_258411.2.
- Whole cell lysate from Hela and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 70 and 80 and 130 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTRIM47 belongs to the TRIM/RBCC family. It contains one B box type zinc finger, one B30.2/SPRY domain and one RING type zinc finger. TRIM47 has low expression in most tissues; higher expression in kidney tubular cells and over expressed in astrocytoma tumor cells.
Cellular localizationCytoplasm. Nucleus
- Gene overexpressed in astrocytoma protein antibody
- GOA antibody
- RING finger protein 100 antibody
All lanes : Anti-TRIM47 antibody (ab72234) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 70 kDa
Observed band size: 70,80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of TRIM47.
Lane 1: ab72234 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72234 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
ab72234 has not yet been referenced specifically in any publications.