Anti-TRIM5 alpha antibody (ab190235)
Key features and details
- Rabbit polyclonal to TRIM5 alpha
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TRIM5 alpha antibody
See all TRIM5 alpha primary antibodies -
Description
Rabbit polyclonal to TRIM5 alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TRIM5 alpha (internal sequence). The exact sequence is proprietary. NM_033034.2.
Database link: Q9C035 -
Positive control
- Human brain cortex tissue: Human stomach cells: Human stomach, thymus and uterus cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
Post-translational
modificationsUbiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro). -
Cellular localization
Cytoplasm > P-body. Cytoplasmic bodies. - Information by UniProt
-
Database links
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
-
Alternative names
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
Images
-
All lanes : Anti-TRIM5 alpha antibody (ab190235) at 2 µg/ml
Lane 1 : Human stomach cell lysate
Lane 2 : Human thymus cell lysate
Lane 3 : Human uterus cell lysates
Predicted band size: 56 kDa -
Immunofluorescent analysis of Human stomach cells labeling TRIM5 alpha with ab190235 at 10 µg/ml.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling TRIM5 alpha with ab190235 at 2 µg/ml.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab190235 has not yet been referenced specifically in any publications.