Key features and details
- Rabbit polyclonal to TRIM5 alpha
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRIM5 alpha antibody
See all TRIM5 alpha primary antibodies
DescriptionRabbit polyclonal to TRIM5 alpha
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TRIM5 alpha (internal sequence). The exact sequence is proprietary. NM_033034.2.
Database link: Q9C035
- Human brain cortex tissue: Human stomach cells: Human stomach, thymus and uterus cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 56 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionIsoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
modificationsUbiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
Cellular localizationCytoplasm > P-body. Cytoplasmic bodies.
- Information by UniProt
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
All lanes : Anti-TRIM5 alpha antibody (ab190235) at 2 µg/ml
Lane 1 : Human stomach cell lysate
Lane 2 : Human thymus cell lysate
Lane 3 : Human uterus cell lysates
Predicted band size: 56 kDa
Immunofluorescent analysis of Human stomach cells labeling TRIM5 alpha with ab190235 at 10 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling TRIM5 alpha with ab190235 at 2 µg/ml.
ab190235 has not yet been referenced specifically in any publications.