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    trim5-alpha-antibody-ab4389.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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Anti-TRIM5 alpha antibody (ab4389)

  • Datasheet
Reviews (2) Submit a question References (7)

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Western blot - Anti-TRIM5 alpha antibody (ab4389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)
  • Western blot - Anti-TRIM5 alpha antibody (ab4389)

Key features and details

  • Goat polyclonal to TRIM5 alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Cat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-TRIM5 alpha antibody
    See all TRIM5 alpha primary antibodies
  • Description

    Goat polyclonal to TRIM5 alpha
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise all three human isoforms.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    ASGILVNVKEEVTC

    , corresponding to N terminal amino acids 2-15 of Human RNF88.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human TRIM5 alpha protein (Tagged) (ab239570)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

A 1 hour primary incubation is recommended for this product.

IHC-P
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

A 1 hour primary incubation is recommended for this product.

IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Post-translational
    modifications

    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • Cellular localization

    Cytoplasm > P-body. Cytoplasmic bodies.
  • Target information above from: UniProt accession Q9C035 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 85363 Human
    • Omim: 608487 Human
    • SwissProt: Q9C035 Human
    • Unigene: 370515 Human
    • Alternative names

      • RING finger protein 88 antibody
      • RNF88 antibody
      • TRIM5 antibody
      • TRIM5_HUMAN antibody
      • TRIM5alpha antibody
      • Tripartite motif containing 5 antibody
      • tripartite motif protein TRIM5 antibody
      • Tripartite motif-containing protein 5 antibody
      see all

    Images

    • Western blot - Anti-TRIM5 alpha antibody (ab4389)
      Western blot - Anti-TRIM5 alpha antibody (ab4389)
      Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      Primary incubation was 1 hour. Detected by chemiluminescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)

      Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

      A) positive cells next to a secondary follicle

      B) positive cells below tonsillar epithelium.

      In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.

    • Western blot - Anti-TRIM5 alpha antibody (ab4389)
      Western blot - Anti-TRIM5 alpha antibody (ab4389)
      Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).

      Predicted band size: 38 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?



      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab4389? Please let us know so that we can cite the reference in this datasheet.

    ab4389 has been referenced in 7 publications.

    • Jimenez-Guardeño JM  et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
    • Singh R  et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). PubMed: 24478420
    • Battivelli E  et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . PubMed: 21632761
    • Neagu MR  et al. Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. J Clin Invest 119:3035-47 (2009). ICC/IF ; Cat . PubMed: 19741300
    • Yap MW  et al. Restriction of foamy viruses by primate Trim5alpha. J Virol 82:5429-39 (2008). WB ; Human . PubMed: 18367529
    • Yap MW  et al. The design of artificial retroviral restriction factors. Virology 365:302-14 (2007). PubMed: 17493656
    • Ohkura S  et al. All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction. J Virol 80:8554-65 (2006). WB ; Human . PubMed: 16912305

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRIM5 alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Glioblastoma cells)
    Permeabilization
    Yes - Triton x-100 0.1%
    Specification
    Glioblastoma cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2019

    Western blot abreview for Anti-TRIM5 alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50000 cells
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2018

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