• Product name
  • Description
    Goat polyclonal to TRIM5 alpha
  • Host species
  • Specificity
    This antibody is expected to recognise all three human isoforms.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Cat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-15 of Human RNF88.

  • Positive control
    • Human placenta, uterus and tonsil lysates.
  • General notes
    Official Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.



Our Abpromise guarantee covers the use of ab4389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution. PubMed: 19741300


  • Function
    Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Post-translational
    Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
  • Cellular localization
    Cytoplasm > P-body. Cytoplasmic bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • RING finger protein 88 antibody
    • RNF88 antibody
    • TRIM5 antibody
    • TRIM5_HUMAN antibody
    • TRIM5alpha antibody
    • Tripartite motif containing 5 antibody
    • tripartite motif protein TRIM5 antibody
    • Tripartite motif-containing protein 5 antibody
    see all


  • Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

    A) positive cells next to a secondary follicle

    B) positive cells below tonsillar epithelium.

    In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.

  • Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size: 38 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


This product has been referenced in:
  • Singh R  et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). Read more (PubMed: 24478420) »
  • Battivelli E  et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . Read more (PubMed: 21632761) »
See all 6 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50000 cells
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

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Submitted Oct 25 2018


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