Anti-TRIM5 alpha antibody (ab4389)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-TRIM5 alpha antibody
See all TRIM5 alpha primary antibodies -
DescriptionGoat polyclonal to TRIM5 alpha
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Host speciesGoat
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SpecificityThis antibody is expected to recognise all three human isoforms.
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Tested applicationsSuitable for: ELISA, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Cat, Human
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Immunogen
Synthetic peptide:
ASGILVNVKEEVTC
, corresponding to N terminal amino acids 2-15 of Human RNF88. -
Positive control
- Human placenta, uterus and tonsil lysates.
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General notesOfficial Gene Symbol - TRIM5. GenBank Accession Number – NP_149023; NP_149083; NP_149084.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab4389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| WB | Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 19741300 |
Target
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FunctionIsoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
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Post-translational
modificationsUbiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro). -
Cellular localizationCytoplasm > P-body. Cytoplasmic bodies.
- Information by UniProt
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Database links
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
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Alternative names
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
Images
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Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 2 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
Predicted band size: 38 kDa
Observed band size: 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM5 alpha antibody (ab4389)Ab4389 (1ug/ml) staining human RNF88 in human tonsil by Immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
A) positive cells next to a secondary follicle
B) positive cells below tonsillar epithelium.
In paraffin embedded Human Tonsil, ab4389 stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptae and weak in germinal centre.
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Western blot - Anti-TRIM5 alpha antibody (ab4389)Anti-TRIM5 alpha antibody (ab4389) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Singh R et al. TRIM5a and TRIM22 are differentially regulated according to HIV-1 infection phase and compartment. J Virol 88:4291-303 (2014). Read more (PubMed: 24478420) »
- Battivelli E et al. Modulation of TRIM5alpha activity in human cells by alternatively spliced TRIM5 isoforms. J Virol 85:7828-35 (2011). WB ; Human . Read more (PubMed: 21632761) »
